Team:Alberta/Notebook/protocols/gel extraction

From 2010.igem.org

(Difference between revisions)
(New page: {{Team:Alberta/Head}} {{Team:Alberta/navbar|project=selected}} {{Team:Alberta/beginLeftSideBar}} {{Team:Alberta/endLeftSideBar}} {{Team:Alberta/beginRightSideBar}} {{Team:Alberta/endRig...)
Line 11: Line 11:
{{Team:Alberta/beginMainContent}}
{{Team:Alberta/beginMainContent}}
-
Protocol 7: Gel Extraction
+
==Gel Extraction==
-
Reagents:
+
===Reagents:===
* Clean scalpel
* Clean scalpel
Line 25: Line 25:
-
Procedure:
+
===Procedure:===
* Place the gel on the transilluminator. Put on face shield and gloves.
* Place the gel on the transilluminator. Put on face shield and gloves.
Line 33: Line 33:
-
[[Team:Alberta/Notebook/protocols| Back]]
+
 
{{Team:Alberta/endMainContent}}
{{Team:Alberta/endMainContent}}

Revision as of 01:33, 27 October 2010

TEAM ALBERTA

Gel Extraction

Reagents:

  • Clean scalpel
  • Transilluminator
  • Eppendorf tube
  • Gel extraction kit
  • 55oC water bath
  • Vortex
  • Microfuge


Procedure:

  • Place the gel on the transilluminator. Put on face shield and gloves.
  • Turn on transilluminator and quickly make four slices; one on each side of the band you want to cut out and turn transilluminator off. Keep exposure to a minimum.
  • Pry out the gel ‘cube’ and place it in an eppendorf tube.
  • Follow instructions on pp. 25 of [http://www.qiagen.com/literature/render.aspx?id=103715 QIAQuick Spin Handbook].