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Team:Calgary/17 September 2010
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| - | + | Friday September 17, 2010| | |
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| + | [[Image:09.17.2010 SSdel-1.jpg|thumb|400px|The first agarose gel electrophoresis of MalEΔSS from the Betton lab plasmids]] | ||
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| + | [[Image:09.17.2010 31SSdel-2.jpg|thumb|400px|The second agarose gel electrophoresis of MalE31ΔSS from the Betton lab plasmids]] | ||
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| + | [[Image:09.17.2010 SSdel-3.jpg|400px|thumb|The third agarose gel electrophoresis of MalEΔSS from the Betton lab plasmids]] | ||
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| + | [[Image:09.17.2010 31SSdel-4.jpg|thumb|400px|The fourth agarose gel electrophopresis of MalE31ΔSS from the Betton lab plasmids]] | ||
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| + | [[Image:09.17.2010 SSdel-5!.jpg|thumb|400px|The fifth gel electrophoresis of MalEΔSS from the Betton lab plasmids]] | ||
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| + | [[Image:09.17.2010 31SSdel-6.jpg|thumb|400px|The sixth gel electrophoresis of MalE31ΔSS from the Betton lab plasmids]] | ||
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| + | [[Image:09.17.2010 ssDEL-7!.jpg|thumb|400px|The seventh gel electrophoresis of MalEΔSS from the Betton lab plasmids]] | ||
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| + | [[Image:09.17.2010 31SSdel-8.jpg|thumb|400px|The eighth gel electrophoresis of MalE31ΔSS from the Betton lab plasmids]] | ||
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| + | [[Image:09.17.2010 SSdel-9.jpg|thumb|400px|The ninth gel electrohporesis of MalEΔSS from the Betton lab plasmids]] | ||
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| + | [[Image:09.17.2010 31SSdel-10!!.jpg|thumb|400px|The tenth gel electrophoresis of MalE31ΔSS from the Betton lab plasmids]] | ||
<u>Himika</u> | <u>Himika</u> | ||
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Today I did a restriction digest of the miniprepped plasmids with EcoRI and PstI. I also induced the CpxR system. Although due to misnomers with the shaker, the induction did not succeed. I will try that again next week. I also looked more into the araC promoter which might help us with our characterization. | Today I did a restriction digest of the miniprepped plasmids with EcoRI and PstI. I also induced the CpxR system. Although due to misnomers with the shaker, the induction did not succeed. I will try that again next week. I also looked more into the araC promoter which might help us with our characterization. | ||
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| + | <u>Chris</u> | ||
| + | Today, Emily and I ran 1.0% agarose gels of each row of Emily's gigantic PCR of malEΔSS and malE31ΔSS. The gels can be seen on the right and it appears that the Pfx polymerase was most successful in bringing out malEΔSS and malE31ΔSS. | ||
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Latest revision as of 00:46, 27 October 2010
Friday September 17, 2010
Himika
Today I did a restriction digest of the miniprepped plasmids with EcoRI and PstI. I also induced the CpxR system. Although due to misnomers with the shaker, the induction did not succeed. I will try that again next week. I also looked more into the araC promoter which might help us with our characterization.
Chris
Today, Emily and I ran 1.0% agarose gels of each row of Emily's gigantic PCR of malEΔSS and malE31ΔSS. The gels can be seen on the right and it appears that the Pfx polymerase was most successful in bringing out malEΔSS and malE31ΔSS.
