The first agarose gel electrophoresis of MalEΔSS from the Betton lab plasmids

The second agarose gel electrophoresis of MalE31ΔSS from the Betton lab plasmids

The third agarose gel electrophoresis of MalEΔSS from the Betton lab plasmids

The fourth agarose gel electrophopresis of MalE31ΔSS from the Betton lab plasmids

The fifth gel electrophoresis of MalEΔSS from the Betton lab plasmids

The sixth gel electrophoresis of MalE31ΔSS from the Betton lab plasmids

The seventh gel electrophoresis of MalEΔSS from the Betton lab plasmids

The eighth gel electrophoresis of MalE31ΔSS from the Betton lab plasmids

The ninth gel electrohporesis of MalEΔSS from the Betton lab plasmids

The tenth gel electrophoresis of MalE31ΔSS from the Betton lab plasmids

Himika

Today I did a restriction digest of the miniprepped plasmids with EcoRI and PstI. I also induced the CpxR system. Although due to misnomers with the shaker, the induction did not succeed. I will try that again next week. I also looked more into the araC promoter which might help us with our characterization.

Chris Today, Emily and I ran 1.0% agarose gels of each row of Emily's gigantic PCR of malEΔSS and malE31ΔSS. The gels can be seen on the right and it appears that the Pfx polymerase was most successful in bringing out malEΔSS and malE31ΔSS.