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Team:Alberta/Notebook/protocols/glycerol
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| - | Protocol | + | ==Protocol: Glycerol Stock== |
| - | Reagents: | + | ===Reagents:=== |
* Overnight with bacterial growth (probably 1.5mL) | * Overnight with bacterial growth (probably 1.5mL) | ||
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| - | Procedure: | + | ===Procedure:=== |
* Pipet 150uL 50% glycerol into 3* properly labeled 1.5 ml screw cap tubes. | * Pipet 150uL 50% glycerol into 3* properly labeled 1.5 ml screw cap tubes. | ||
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| - | Notes: | + | ===Notes:=== |
* If we make extra we have them if we need to send them out or want to store in several locations. | * If we make extra we have them if we need to send them out or want to store in several locations. | ||
Revision as of 00:18, 27 October 2010
Protocol: Glycerol Stock
Reagents:
- Overnight with bacterial growth (probably 1.5mL)
- Screw cap tubes
- Glycerol
Procedure:
- Pipet 150uL 50% glycerol into 3* properly labeled 1.5 ml screw cap tubes.
- Add 350uL of overnight culture to each tube.
- Pipet up and down to gently mix.
- Flash freeze in liquid N2 or dry ice/methanol bath.
- Place in -80oC freezer when frozen.
Notes:
- If we make extra we have them if we need to send them out or want to store in several locations.
