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Team:Harvard/flavor/parts
From 2010.igem.org
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<tr><td><b>Name</b></td><td><b>Description</b></td><td><b>Sequence</b></td></tr> | <tr><td><b>Name</b></td><td><b>Description</b></td><td><b>Sequence</b></td></tr> | ||
| - | <tr><td>J45004_F</td><td>Wintergreen pathway part. Encodes SAM benzoic acid/salicylic acid carboxyl methyltransferase I. Forward primer</td><td></td></tr> | + | <tr><td>J45004_F</td><td>Wintergreen pathway part. Encodes SAM benzoic acid/salicylic acid carboxyl methyltransferase I. Forward primer</td><td><a href="https://2010.igem.org/Team:Harvard/J45004_F">link</a></td></tr> |
| - | <tr><td>J45004_R</td><td>Wintergreen pathway part. Encodes SAM benzoic acid/salicylic acid carboxyl methyltransferase I. Reverse primer</td><td></td></tr> | + | <tr><td>J45004_R</td><td>Wintergreen pathway part. Encodes SAM benzoic acid/salicylic acid carboxyl methyltransferase I. Reverse primer</td><td><a href="https://2010.igem.org/Team:Harvard/J45004_R">link</a></td></tr> |
| - | <tr><td>J45017_F</td><td>Wintergreen pathway part. Encodes an isochorismate pyruvate-lyase and an isochorismate synthase. Forward primer</td><td></td></tr> | + | <tr><td>J45017_F</td><td>Wintergreen pathway part. Encodes an isochorismate pyruvate-lyase and an isochorismate synthase. Forward primer</td><td><a href="https://2010.igem.org/Team:Harvard/J45017_F">link</a></td></tr> |
| - | <tr><td>J45017_R</td><td>Wintergreen pathway part. Encodes an isochorismate pyruvate-lyase and an isochorismate synthase. Reverse primer</td><td></td></tr> | + | <tr><td>J45017_R</td><td>Wintergreen pathway part. Encodes an isochorismate pyruvate-lyase and an isochorismate synthase. Reverse primer</td><td><a href="https://2010.igem.org/Team:Harvard/J45017_R">link</a></td></tr> |
| - | <tr><td>NOSterm_BB_F</td><td>Plant specific terminator forward primer</td><td></td></tr> | + | <tr><td>NOSterm_BB_F</td><td>Plant specific terminator forward primer</td><td><a href="https://2010.igem.org/Team:Harvard/NOSterm_BB_F">link</a></td></tr> |
| - | <tr><td>NOSterm_BB_R</td><td>Plant specific terminator reverse primer</td><td></td></tr> | + | <tr><td>NOSterm_BB_R</td><td>Plant specific terminator reverse primer</td><td><a href="https://2010.igem.org/Team:Harvard/NOSterm_BB_R">link</a></td></tr> |
| - | <tr><td>Stop_NOSterm_BB_F</td><td>NOSt plant specific terminator with stop codon on end</td><td></td></tr> | + | <tr><td>Stop_NOSterm_BB_F</td><td>NOSt plant specific terminator with stop codon on end</td><td><a href="https://2010.igem.org/Team:Harvard/Stop_NOSterm_BB_F">link</a></td></tr> |
| - | <tr><td>pENTCUP2_BB_F</td><td>Plant specific promoter forward primer</td><td></td></tr> | + | <tr><td>pENTCUP2_BB_F</td><td>Plant specific promoter forward primer</td><td><a href="https://2010.igem.org/Team:Harvard/pENTCUP2_BB_F">link</a></td></tr> |
| - | <tr><td>pENTCUP2_BB_R</td><td>Plant specific promoter reverse primer</td><td></td></tr> | + | <tr><td>pENTCUP2_BB_R</td><td>Plant specific promoter reverse primer</td><td><a href="https://2010.igem.org/Team:Harvard/pENTCUP2_BB_R">link</a></td></tr> |
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| + | </style> | ||
| + | <div id="maincontent"> | ||
| + | <div id="abstract"> | ||
| + | <h2>Parts</h2> | ||
| + | <table cellspacing="0"> | ||
| + | <tr><td><b>Name</b></td><td><b>Description</b></td><td><b>Length</b></td><td><b>Sequence</b></td></tr> | ||
| + | <tr><td>BBa_K382020</td><td><i>Arabidopsis</i> optimized Brazzein</td><td>195</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382020">link</a></td></tr> | ||
| + | <tr><td>BBa_K382021</td><td><i>Arabidopsis</i> optimized Miraculin</td><td>660</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382021">link</a></td></tr> | ||
| + | <tr><td>BBa_K382022</td><td>pENTCUP2 plant specific promoter</td><td>503</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382022">link</a></td></tr> | ||
| + | <tr><td>BBa_K382023</td><td>NosT plant specific terminator</td><td>253</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382023">link</a></td></tr> | ||
| + | <tr><td>BBa_K382024</td><td>NosT plant specific terminator with STOP codon</td><td>256</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382024">link</a></td></tr> | ||
| + | <tr><td>BBa_K382025</td><td><i>Arabidopsis</i> optimized Miraculin with StrepII N-terminus tag</td><td>696</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382025">link</a></td></tr> | ||
| + | <tr><td>BBa_K382026</td><td><i>Arabidopsis</i> optimized Brazzein with StrepII N-terminus tag</td><td>201</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382026">link</a></td></tr> | ||
| + | <tr><td>BBa_K382027</td><td><i>Arabidopsis</i> optimized Miraculin with StrepII C-terminus tag</td><td>696</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382027">link</a></td></tr> | ||
| + | <tr><td>BBa_K382028</td><td><i>Arabidopsis</i> optimized Brazzein with StrepII C-terminus tag</td><td>201</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382028">link</a></td></tr> | ||
| + | |||
| + | |||
| + | </table> | ||
| + | |||
| + | </div> | ||
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Latest revision as of 00:15, 27 October 2010
parts and primers
Primers
| Name | Description | Sequence |
| J45004_F | Wintergreen pathway part. Encodes SAM benzoic acid/salicylic acid carboxyl methyltransferase I. Forward primer | link |
| J45004_R | Wintergreen pathway part. Encodes SAM benzoic acid/salicylic acid carboxyl methyltransferase I. Reverse primer | link |
| J45017_F | Wintergreen pathway part. Encodes an isochorismate pyruvate-lyase and an isochorismate synthase. Forward primer | link |
| J45017_R | Wintergreen pathway part. Encodes an isochorismate pyruvate-lyase and an isochorismate synthase. Reverse primer | link |
| NOSterm_BB_F | Plant specific terminator forward primer | link |
| NOSterm_BB_R | Plant specific terminator reverse primer | link |
| Stop_NOSterm_BB_F | NOSt plant specific terminator with stop codon on end | link |
| pENTCUP2_BB_F | Plant specific promoter forward primer | link |
| pENTCUP2_BB_R | Plant specific promoter reverse primer | link |
Parts
| Name | Description | Length | Sequence |
| BBa_K382020 | Arabidopsis optimized Brazzein | 195 | link |
| BBa_K382021 | Arabidopsis optimized Miraculin | 660 | link |
| BBa_K382022 | pENTCUP2 plant specific promoter | 503 | link |
| BBa_K382023 | NosT plant specific terminator | 253 | link |
| BBa_K382024 | NosT plant specific terminator with STOP codon | 256 | link |
| BBa_K382025 | Arabidopsis optimized Miraculin with StrepII N-terminus tag | 696 | link |
| BBa_K382026 | Arabidopsis optimized Brazzein with StrepII N-terminus tag | 201 | link |
| BBa_K382027 | Arabidopsis optimized Miraculin with StrepII C-terminus tag | 696 | link |
| BBa_K382028 | Arabidopsis optimized Brazzein with StrepII C-terminus tag | 201 | link |
