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Team:Calgary/22 June 2010
From 2010.igem.org
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'''Tuesday June 22, 2010''' | '''Tuesday June 22, 2010''' | ||
| - | + | Patrick: Being the highly intelligent person that I was, I realized that yesterday I had constructed the R0040 onto the original E0430, which was on a pSB1A2 plasmid. I plated on kanamycin agar plates. Thus, no growth. I will retry the construction once more using the plasmid-switched E0430. We also did a miniprep of E0420. | |
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| + | I also began the screening of the ''cpxP'' promoter. When we had designed the primers to PCR out the promoter from genomic DNA, we had added ''XbaI'' and ''SpeI'' sites to the primers, which are non-directional and may scar. Thus, we will try and digest 10 trials of ''cpxP'' over the next few days with these two enzymes, and hopefully pick out those that do separate into separate bands in a gel. | ||
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Revision as of 18:36, 7 July 2010
Tuesday June 22, 2010
Patrick: Being the highly intelligent person that I was, I realized that yesterday I had constructed the R0040 onto the original E0430, which was on a pSB1A2 plasmid. I plated on kanamycin agar plates. Thus, no growth. I will retry the construction once more using the plasmid-switched E0430. We also did a miniprep of E0420.
I also began the screening of the cpxP promoter. When we had designed the primers to PCR out the promoter from genomic DNA, we had added XbaI and SpeI sites to the primers, which are non-directional and may scar. Thus, we will try and digest 10 trials of cpxP over the next few days with these two enzymes, and hopefully pick out those that do separate into separate bands in a gel.
No notebook page exists for this date. Sorry!
