Team:Brown/Protocols

From 2010.igem.org

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[[Image:pipette_macro.jpg|right|400px]]
[[Image:pipette_macro.jpg|right|400px]]
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PCR
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'''PCR'''
*[[:Team:Brown/Protocols/PCR|PCR]]
*[[:Team:Brown/Protocols/PCR|PCR]]
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PCR Cleanup/Purification
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'''PCR Cleanup/Purification'''
*[[Media:QIAquick_Spin_Handbook.pdf|PCR Cleanup/Purification PDF (page 19)]]
*[[Media:QIAquick_Spin_Handbook.pdf|PCR Cleanup/Purification PDF (page 19)]]
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Gel Electrophoresis
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'''Gel Electrophoresis'''
*[[:Team:Brown/Protocols/Gels|Agarose gels]]
*[[:Team:Brown/Protocols/Gels|Agarose gels]]
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Gel Extraction
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'''Gel Extraction'''
*[[Media:QIAquick_Spin_Handbook.pdf|Gel Extraction PDF (page 25)]]
*[[Media:QIAquick_Spin_Handbook.pdf|Gel Extraction PDF (page 25)]]
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Ligations
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'''Ligations'''
*[[:Team:Brown/Protocols/Ligation|Ligation]]
*[[:Team:Brown/Protocols/Ligation|Ligation]]
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Restriction digests
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'''Restriction digests'''
*[[:Team:Brown/Protocols/Restriction_Digest|Restriction [Double] Digest]]
*[[:Team:Brown/Protocols/Restriction_Digest|Restriction [Double] Digest]]
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Overnights
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'''Overnights'''
*[[:Team:Brown/Protocols/Overnight_Culture|Overnight Culture]]
*[[:Team:Brown/Protocols/Overnight_Culture|Overnight Culture]]
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Transformations
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'''Transformations'''
*[[:Team:Brown/Protocols/Transformation|Bacterial Cell Transformation]]
*[[:Team:Brown/Protocols/Transformation|Bacterial Cell Transformation]]
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Cell competence protocols
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'''Cell competence protocols'''
*[[Media:Protocol_Quick_CaCl2_competence.pdf|Quick CaCL2 Competence PDF]]
*[[Media:Protocol_Quick_CaCl2_competence.pdf|Quick CaCL2 Competence PDF]]
*[[Media:Protocol_CaCl2_competent_cells.pdf|CaCl2 competence (bulk) PDF]]
*[[Media:Protocol_CaCl2_competent_cells.pdf|CaCl2 competence (bulk) PDF]]
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Minipreps
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'''Minipreps'''
*[[Media:QIAprep_Miniprep_Handbook.pdf|Miniprep handbook PDF (pages 22-23)]]
*[[Media:QIAprep_Miniprep_Handbook.pdf|Miniprep handbook PDF (pages 22-23)]]
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[[Image:centrifuge_spinning.JPG]]
 

Revision as of 21:42, 26 October 2010

Protocols

Here are the protocols that we followed. Occasionally we make exceptions to the general "recipe," but those exceptions are documented in our Notebook.

Pipette macro.jpg

PCR

PCR Cleanup/Purification

Gel Electrophoresis

Gel Extraction

Ligations

Restriction digests

Overnights

Transformations

Cell competence protocols

Minipreps