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Team:Brown/Protocols
From 2010.igem.org
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[[Image:pipette_macro.jpg|right|400px]] | [[Image:pipette_macro.jpg|right|400px]] | ||
| - | PCR | + | '''PCR''' |
*[[:Team:Brown/Protocols/PCR|PCR]] | *[[:Team:Brown/Protocols/PCR|PCR]] | ||
| - | PCR Cleanup/Purification | + | '''PCR Cleanup/Purification''' |
*[[Media:QIAquick_Spin_Handbook.pdf|PCR Cleanup/Purification PDF (page 19)]] | *[[Media:QIAquick_Spin_Handbook.pdf|PCR Cleanup/Purification PDF (page 19)]] | ||
| - | Gel Electrophoresis | + | '''Gel Electrophoresis''' |
*[[:Team:Brown/Protocols/Gels|Agarose gels]] | *[[:Team:Brown/Protocols/Gels|Agarose gels]] | ||
| - | Gel Extraction | + | '''Gel Extraction''' |
*[[Media:QIAquick_Spin_Handbook.pdf|Gel Extraction PDF (page 25)]] | *[[Media:QIAquick_Spin_Handbook.pdf|Gel Extraction PDF (page 25)]] | ||
| - | Ligations | + | '''Ligations''' |
*[[:Team:Brown/Protocols/Ligation|Ligation]] | *[[:Team:Brown/Protocols/Ligation|Ligation]] | ||
| - | Restriction digests | + | '''Restriction digests''' |
*[[:Team:Brown/Protocols/Restriction_Digest|Restriction [Double] Digest]] | *[[:Team:Brown/Protocols/Restriction_Digest|Restriction [Double] Digest]] | ||
| - | Overnights | + | '''Overnights''' |
*[[:Team:Brown/Protocols/Overnight_Culture|Overnight Culture]] | *[[:Team:Brown/Protocols/Overnight_Culture|Overnight Culture]] | ||
| - | Transformations | + | '''Transformations''' |
*[[:Team:Brown/Protocols/Transformation|Bacterial Cell Transformation]] | *[[:Team:Brown/Protocols/Transformation|Bacterial Cell Transformation]] | ||
| - | Cell competence protocols | + | '''Cell competence protocols''' |
*[[Media:Protocol_Quick_CaCl2_competence.pdf|Quick CaCL2 Competence PDF]] | *[[Media:Protocol_Quick_CaCl2_competence.pdf|Quick CaCL2 Competence PDF]] | ||
*[[Media:Protocol_CaCl2_competent_cells.pdf|CaCl2 competence (bulk) PDF]] | *[[Media:Protocol_CaCl2_competent_cells.pdf|CaCl2 competence (bulk) PDF]] | ||
| - | Minipreps | + | '''Minipreps''' |
*[[Media:QIAprep_Miniprep_Handbook.pdf|Miniprep handbook PDF (pages 22-23)]] | *[[Media:QIAprep_Miniprep_Handbook.pdf|Miniprep handbook PDF (pages 22-23)]] | ||
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Revision as of 21:42, 26 October 2010
Protocols
Here are the protocols that we followed. Occasionally we make exceptions to the general "recipe," but those exceptions are documented in our Notebook.
PCR
PCR Cleanup/Purification
Gel Electrophoresis
Gel Extraction
Ligations
Restriction digests
Overnights
Transformations
Cell competence protocols
Minipreps
