Team:Brown/Protocols

From 2010.igem.org

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==Protocols==
==Protocols==
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Here are the protocols that we followed. Occasionally we make exceptions to the general "recipe," but those exceptions are documented in our [[:Team:Brown/Notebook|Notebook]].
Here are the protocols that we followed. Occasionally we make exceptions to the general "recipe," but those exceptions are documented in our [[:Team:Brown/Notebook|Notebook]].
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Minipreps
Minipreps
*[[Media:QIAprep_Miniprep_Handbook.pdf|Miniprep handbook PDF (pages 22-23)]]
*[[Media:QIAprep_Miniprep_Handbook.pdf|Miniprep handbook PDF (pages 22-23)]]
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Revision as of 21:39, 26 October 2010

Protocols

Here are the protocols that we followed. Occasionally we make exceptions to the general "recipe," but those exceptions are documented in our Notebook.

PCR

PCR Cleanup/Purification

Gel Electrophoresis

Gel Extraction

Ligations

Restriction digests

Overnights

Transformations

Cell competence protocols

Minipreps

Centrifuge spinning.JPG