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Team:UPO-Sevilla/Notebook/09 10
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| - | <h1>September, | + | <h1>September, 10th</h1> |
<h2>Production Team</h2> | <h2>Production Team</h2> | ||
| - | <p | + | <p> We changed some conditions to improve our PCR results:</p> |
<ul> | <ul> | ||
| Line 27: | Line 27: | ||
</ul> | </ul> | ||
| - | <p>0’8% gel electrophoresis analysis showed a line of 1Kb, which is the length of <i>fecR</i>. There was also a 0’2Kb line. It looked like that the new conditions carried us to the goal. We had to isolate from gel the new part | + | <p>0’8% gel electrophoresis analysis showed a line of 1Kb, which is the length of <i>fecR</i>. There was also a 0’2Kb line. It looked like that the new conditions carried us to the goal. We had to isolate from gel the new part: WE GOT FECR!</p> |
<h2>Assembly Team</h2> | <h2>Assembly Team</h2> | ||
<p>We realized a new ligation of the failed biobricks and digested not available biobricks (2+6) and vectors (pSB1C3 and pSB1T3).</p> | <p>We realized a new ligation of the failed biobricks and digested not available biobricks (2+6) and vectors (pSB1C3 and pSB1T3).</p> | ||
| + | |||
| + | <h2>Assay Team</h2> | ||
| + | |||
| + | <p>We developed different assays by changing different features; the plates were incubated overnight.</p> | ||
| + | |||
| + | <a class="return_button" href="/Team:UPO-Sevilla/Notebook/09_09" title="Go to 9th of September"><span>Go to 9th of September</span></a> | ||
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| + | <a class="next_button" href="/Team:UPO-Sevilla/Notebook/09_13" title="Go to 13th of September"><span>Go to 13th of September</span></a> | ||
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| + | <div class="clear"></div> | ||
<a class="return_button" href="/Team:UPO-Sevilla/Notebook" title="Notebook"><span>Return to Notebook</span></a> | <a class="return_button" href="/Team:UPO-Sevilla/Notebook" title="Notebook"><span>Return to Notebook</span></a> | ||
Latest revision as of 20:59, 26 October 2010
September, 10th
Production Team
We changed some conditions to improve our PCR results:
- We used new Pfu enzyme, buffer and dNTP.
- Templates: we diluted the high concentrated fragment (1/10) and used 2ul instead of 1ul of the low concentrated one.
0’8% gel electrophoresis analysis showed a line of 1Kb, which is the length of fecR. There was also a 0’2Kb line. It looked like that the new conditions carried us to the goal. We had to isolate from gel the new part: WE GOT FECR!
Assembly Team
We realized a new ligation of the failed biobricks and digested not available biobricks (2+6) and vectors (pSB1C3 and pSB1T3).
Assay Team
We developed different assays by changing different features; the plates were incubated overnight.
Go to 9th of September Go to 13th of September Return to Notebook
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