Team:Uppsala-SwedenWeek16

From 2010.igem.org

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(New page: {{Template:Uppsala}}<!--Do not remove the first and last lines in this page!--> == Week-16 ==)
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{{Template:Uppsala}}<!--Do not remove the first and last lines in this page!-->
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== Week-16 ==
== Week-16 ==
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Start to apply high fidelity PCR due the construct are getting larger and the regular Taq polymerase may not perform as well as the Phusion Polymerase at this length. Based on the company´s manual, we calculated the
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<TABLE WIDTH=405 BORDER=1 BORDERCOLOR="#000000" CELLPADDING=7 CELLSPACING=0>
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<COL WIDTH=106>
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<COL WIDTH=81>
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<COL WIDTH=80>
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<COL WIDTH=80>
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<TR VALIGN=TOP>
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<TD WIDTH=106>
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<P LANG="sv-SE" CLASS="western"><BR>
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</P>
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</TD>
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<TD WIDTH=81>
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<P LANG="sv-SE" CLASS="western">Temperatur</P>
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</TD>
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<TD WIDTH=80>
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<P LANG="sv-SE" CLASS="western">Time</P>
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</TD>
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<TD WIDTH=80>
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<P LANG="sv-SE" CLASS="western">Cycle</P>
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</TD>
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</TR>
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<TR VALIGN=TOP>
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<TD WIDTH=106>
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<P CLASS="western"><SPAN LANG="sv-SE">Initial</SPAN><SPAN LANG="sv-SE">
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denaturation</SPAN></P>
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</TD>
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<TD WIDTH=81>
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<P LANG="sv-SE" CLASS="western">98</P>
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</TD>
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<TD WIDTH=80>
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<P LANG="sv-SE" CLASS="western">30 s
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</P>
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</TD>
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<TD WIDTH=80>
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<P LANG="sv-SE" CLASS="western">1</P>
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</TD>
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</TR>
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<TR VALIGN=TOP>
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<TD WIDTH=106>
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<P LANG="sv-SE" CLASS="western">Denaturation</P>
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</TD>
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<TD WIDTH=81>
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<P LANG="sv-SE" CLASS="western">98</P>
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</TD>
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<TD WIDTH=80>
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<P LANG="sv-SE" CLASS="western">10</P>
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</TD>
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<TD WIDTH=80>
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<P LANG="sv-SE" CLASS="western">30</P>
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</TD>
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</TR>
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<TR VALIGN=TOP>
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<TD WIDTH=106>
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<P CLASS="western"><SPAN LANG="sv-SE">Anne</SPAN><SPAN LANG="sv-SE">aling</SPAN></P>
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</TD>
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<TD WIDTH=81>
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<P LANG="sv-SE" CLASS="western">55.3</P>
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</TD>
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<TD WIDTH=80>
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<P LANG="sv-SE" CLASS="western">10s</P>
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</TD>
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<TD WIDTH=80>
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<P LANG="sv-SE" CLASS="western">30</P>
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</TD>
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</TR>
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<TR VALIGN=TOP>
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<TD WIDTH=106>
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<P LANG="sv-SE" CLASS="western">Extension</P>
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</TD>
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<TD WIDTH=81>
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<P LANG="sv-SE" CLASS="western">72</P>
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</TD>
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<TD WIDTH=80>
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<P LANG="sv-SE" CLASS="western">1 min 45 s</P>
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</TD>
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<TD WIDTH=80>
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<P LANG="sv-SE" CLASS="western">30</P>
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</TD>
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</TR>
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<TR VALIGN=TOP>
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<TD WIDTH=106>
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<P CLASS="western"><SPAN LANG="sv-SE">Final ex</SPAN><SPAN LANG="sv-SE">tension</SPAN></P>
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</TD>
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<TD WIDTH=81>
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<P LANG="sv-SE" CLASS="western">72</P>
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</TD>
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<TD WIDTH=80>
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<P LANG="sv-SE" CLASS="western">5 min</P>
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</TD>
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<TD WIDTH=80>
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<P LANG="sv-SE" CLASS="western">1</P>
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</TD>
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</TR>
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<TR VALIGN=TOP>
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<TD WIDTH=106>
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<P LANG="sv-SE" CLASS="western"><BR>
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</P>
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</TD>
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<TD WIDTH=81>
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<P LANG="sv-SE" CLASS="western">4</P>
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</TD>
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<TD WIDTH=80>
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<P LANG="sv-SE" CLASS="western">forever</P>
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</TD>
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<TD WIDTH=80>
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<P LANG="sv-SE" CLASS="western"><BR>
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</P>
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</TD>
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</TR>
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</TABLE>

Revision as of 19:39, 26 October 2010

Week-16

Start to apply high fidelity PCR due the construct are getting larger and the regular Taq polymerase may not perform as well as the Phusion Polymerase at this length. Based on the company´s manual, we calculated the

<COL WIDTH=106> <COL WIDTH=81> <COL WIDTH=80> <COL WIDTH=80>


Temperatur

Time

Cycle

Initial denaturation

98

30 s

1

Denaturation

98

10

30

Annealing

55.3

10s

30

Extension

72

1 min 45 s

30

Final extension

72

5 min

1


4

forever


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