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Team:Tsinghua/Notebook/21 August 2010
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(New page: == Module I, group 2c == == Module I, group 2c == Extract plasmid from the clone we Picked yesterday. Digest the plasmid with EcoRI and run gel to identify whether the clone is a positiv...) |
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== Module I, group 2c == | == Module I, group 2c == | ||
Extract plasmid from the clone we Picked yesterday. | Extract plasmid from the clone we Picked yesterday. | ||
Latest revision as of 19:34, 26 October 2010
Module I, group 2c
Extract plasmid from the clone we Picked yesterday. Digest the plasmid with EcoRI and run gel to identify whether the clone is a positive one.
digestion system
plasmid 6ul EcoRI 1ul FD Buffer 2ul H2O 11ul
Result: None of the clone picked is positive.
Analysis: Since no positive clones have been found, we come to check the principle and design of our experiment. We realize that if we use AP to treat with inserted fragments and vector, it is obvious that ligation can not talk place.
