Team:Heidelberg/Modeling/miBSdesigner

From 2010.igem.org

(Difference between revisions)
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<center>
<center>
<div class="t1">
<div class="t1">
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Construction of microRNA binding sites </div><br><br>
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Construction of microRNA binding sites </div><br>
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<div class="largetext">
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<div style="width:350px; text-align:right;"><span class="help"><a href="#">?</a><span></div>
 +
<div class="largetext" style="width:450px;">
Sequence name<br>
Sequence name<br>
<input type="text" name="name" id="name" size="40" value="hsa-miR122"/><br>
<input type="text" name="name" id="name" size="40" value="hsa-miR122"/><br>
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Spacer (inert sequence)<br>
Spacer (inert sequence)<br>
<input type="text" name="spacer" id="spacer" size="40" value="TTATATTTTATGACA"/>
<input type="text" name="spacer" id="spacer" size="40" value="TTATATTTTATGACA"/>
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<span class="help"><a href="#">?</a></span></div></div></center>
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</center><br><br>
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<br><br>
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<div class="largetext" style="width:400px;">
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<div class="largetext">
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<input name="quality" value="perfect" onchange="chMd()" checked="checked" type="radio">
<input name="quality" value="perfect" onchange="chMd()" checked="checked" type="radio">
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Perfect Binding Site<br>
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Perfect Binding Site <span class="help" style="float:right;"><a href="#">?</a></span><br>
<input name="quality" value="bulge" onchange="chMd()" type="radio">
<input name="quality" value="bulge" onchange="chMd()" type="radio">
Imperfect Binding Site with 4 nt bulge (9-12)<br>
Imperfect Binding Site with 4 nt bulge (9-12)<br>
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<table>
<table>
<tr height=120px>
<tr height=120px>
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<td width=320px>
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<td width=290px>
<div class="title">
<div class="title">
Seed sequence (1-8) &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; <span class="help"><a href="#">?</a></span>
Seed sequence (1-8) &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; <span class="help"><a href="#">?</a></span>

Revision as of 19:08, 26 October 2010

BS designer for miRNA
Construction of microRNA binding sites

Sequence name

microRNA Sequence ( 5' -> 3')

Spacer (inert sequence)


Perfect Binding Site ?
Imperfect Binding Site with 4 nt bulge (9-12)
Personalized Binding Site

Seed sequence (1-8)              ?
Customized MISMATCH position
Supplementary region        ?
Customized MATCH positions
Modify AU content                ?
A in position -1
A in position 1
A in position 9
A in position 0
A in position 8
A in position 10
Sticky ends for integration into plasmid   ?
Customized:
5'  
3'  



Name of primer 1


Sequence of primer 1


Name of primer 2


Sequence of primer 2