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Team:Uppsala-SwedenWeek12
From 2010.igem.org
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[[Image:20100817 H3--H4 c-PCR 20100814.jpg|500px|thumb|center|border|H3-H4]] | [[Image:20100817 H3--H4 c-PCR 20100814.jpg|500px|thumb|center|border|H3-H4]] | ||
| - | [[Image:20100817 H5-H6 c-PCR 20100814(3).jpg|500px|thumb|center|border| | + | [[Image:20100817 H5-H6 c-PCR 20100814(3).jpg|500px|thumb|center|border|H5-H6]] |
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| + | The samples selected in Colony PCR were inoculated for obtaining material to do plasmid extraction. Overnight culture was used to make glycerol stocks and plasmids were extracted. The plasmid concentration was measured for estimating the amount of enzymes required for digestion and ligation. | ||
Revision as of 17:55, 26 October 2010
Week-12
Construction of H1- G8
5 colonies were picked from each of those plates and sent to perform c-PCR. According to the band size on gel picture, 1 or 2 colonies of each set were selected to inoculate.
From the above gel picture it was not very clear. Some fiddling around with the controls and we got a much better image (given below).
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The samples selected in Colony PCR were inoculated for obtaining material to do plasmid extraction. Overnight culture was used to make glycerol stocks and plasmids were extracted. The plasmid concentration was measured for estimating the amount of enzymes required for digestion and ligation.
