Team:Yale/Our Project/Notebook

From 2010.igem.org

(Difference between revisions)
Line 76: Line 76:
     <li>12 concentrations of copper(II) sulfate/LB solution were used with final (post-E. coli addition) copper(II) sulfate concentrations of 1 M, 0.1 M, 50 mM, 10 mM, 1 mM, 500 uM, 250 uM, 100 uM, 10 uM, 1 uM, 0.1 uM, and 0 uM, and each concentration was given its own column in the well plate. A row of cells with just the copper solutions was included to function as blanks </li>
     <li>12 concentrations of copper(II) sulfate/LB solution were used with final (post-E. coli addition) copper(II) sulfate concentrations of 1 M, 0.1 M, 50 mM, 10 mM, 1 mM, 500 uM, 250 uM, 100 uM, 10 uM, 1 uM, 0.1 uM, and 0 uM, and each concentration was given its own column in the well plate. A row of cells with just the copper solutions was included to function as blanks </li>
</ul>
</ul>
-
<div padding-top=10px;>
+
<br />
 +
<div>
<img src="https://static.igem.org/mediawiki/2010/3/30/Yale-well-plate.jpg" />
<img src="https://static.igem.org/mediawiki/2010/3/30/Yale-well-plate.jpg" />
<div id="caption">layout of 96-well plate</div>
<div id="caption">layout of 96-well plate</div>

Revision as of 16:47, 26 October 2010

iGEM Yale

lab notebook: week 1 (6/7 - 6/13)

  • Monday 6/7--We successfully defend our project to our sponsors(yay!)
  • Wednesday 6/9--First day in lab!--got set up & started cultures of two E. coli strains
  • See more/less
  • Thursday 6/10--inoculated liquid cultures in AM, made up different copper sulfate & LB solutions, then ran an assay measuring bacterial growth in the presence of copper, trying both strains at three different initial ODs and measuring for four hours
  • See more/less
  • Friday 6/11--Ran another growth assay with middling concentrations based on results of first, but bacteria failed to grow well--perhaps because allowed to overgrow prior to assay?
  • See more/less