Team:Imperial College London/Modelling/Signalling/Detailed Description

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Revision as of 15:46, 26 October 2010

Department of Bioengineering

Division of Molecular Biosciences

Modelling	Overview \| Detection Model \| Signaling Model \| Fast Response Model \| Interactions
A major part of the project consisted of modelling each module. This enabled us to decide which ideas we should implement. Look at the Fast Response page for a great example of how modelling has made a major impact on our design!

Objectives | Description | Results | Constants | MATLAB Code

Detailed Description

We are assuming steady-state for AIP, Phosphate, ComD and ComE in our cell/in the solution. Hence, we can neglect production and degradation rates of ComD and ComE.

Overview of the signalling module

Equation 1: AIP binds to ComD receptor to form a complex (AIP-ComD)

AIP + ComD ↔ AIP-ComD

Equation 2: Phosphate binds to the AIP-ComD complex to form another complex (AIP-ComD*)

AIP-ComD + Phosphate ↔ AIP-ComD*

Equation 3: ComE binds to the AIP-ComD* recepetor to form another complex (AIP-ComD*-ComE)

AIP-ComD* + ComE ↔ AIP-ComD*-ComE

Equation 4: Phosphate group on ComD binds to ComE and forms two products: phosphorylated ComE (ComE*) and AIP-ComD

AIP-ComD*-ComE ↔ AIP-ComD + ComE*

Using the Law of Mass Action, we can rewrite these 4 equations:

In the above equations, the forwards reaction constants are represented by k_1,2,3,4, for the first, second, third and fourth reaction, respectively. The backwards reaction constants are represented by k_-1,-2,-3,-4.

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