Latest revision as of 14:35, 26 October 2010

Growing an Overnight Culture

Transfer 5 ml of LB or appropriate media into a universal tube.
Heat the innoculation loop till red hot to sterilize the loop.
Allow the innoculation loop to cool down for approximately 30 seconds.
The innoculation loop is then used to pick a single colony of bacteria on the agar plate.
Innoculate the LB or appropriate media with the bacteria.
Resterilize the loop.
The culture is then left overnight at 37°C in a shaking incubator at 200 rpm.

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 {{Team:Newcastle/mainbanner}}
-Add 5ml LB broth
+=Growing an Overnight Culture=
-Leave over night at 37°C (''E.coli'', ''B.subtilis'' lower temp)
+==Materials Required==
+* LB Broth
+* Colonies of bacteria on plate
+* Innoculation loop
+* Universal tube
+* Pipette
+==Procedures==
+# Transfer 5 ml of LB or appropriate media into a universal tube.
+# Heat the innoculation loop till red hot to sterilize the loop.
+# Allow the innoculation loop to cool down for approximately 30 seconds.
+# The innoculation loop is then used to pick a single colony of bacteria on the agar plate.
+# Innoculate the LB or appropriate media with the bacteria.
+# Resterilize the loop.
+# The culture is then left overnight at 37°C in a shaking incubator at 200 rpm.
+'''Go back to our [[Team:Newcastle/Protocol list|Protocol List]]'''
+{{Team:Newcastle/footer}}