Latest revision as of 14:35, 26 October 2010

Growing an Overnight Culture

Transfer 5 ml of LB or appropriate media into a universal tube.
Heat the innoculation loop till red hot to sterilize the loop.
Allow the innoculation loop to cool down for approximately 30 seconds.
The innoculation loop is then used to pick a single colony of bacteria on the agar plate.
Innoculate the LB or appropriate media with the bacteria.
Resterilize the loop.
The culture is then left overnight at 37°C in a shaking incubator at 200 rpm.

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 ==Procedures==
-# 5ml of LB broth is pipetted with the help of an auto pipette into a universal tube.
+# Transfer 5 ml of LB or appropriate media into a universal tube.
 # Heat the innoculation loop till red hot to sterilize the loop.
 # Allow the innoculation loop to cool down for approximately 30 seconds.
-# The innoculation loop is then used to pick a colony of the bacteria present on the agar plate.
+# The innoculation loop is then used to pick a single colony of bacteria on the agar plate.
-# The colony is then mixed into the LB Broth in the universal tube.
+# Innoculate the LB or appropriate media with the bacteria.
 # Resterilize the loop.
-# The culture is then left overnight at 37°C in an incubator.
+# The culture is then left overnight at 37°C in a shaking incubator at 200 rpm.