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Team:Newcastle/Agar plates
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Revision as of 14:12, 26 October 2010
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LB agar plates
Materials required
The materials mentioned below is used to make up 1 liter of the standard LB agar plates:
- 10 g of tryptoptan
- 5 g of yeast
- 5 g of NaCl
- 15 g of agar
The materials mentioned is used to make up 1 liter of LB starch agar plates:
- 10 g of tryptoptan
- 5 g of yeast
- 5 g of NaCl
- 15 g of agar
- 10 g of starch
Protocol
- Dissolve the materials in 800 ml of distilled water. ( Note : The agar will not dissolve in distill water. )
- After most of the materials are dissolved, top up the finial volume to 1 liter using distilled water.
- Autoclave the mixture.
- After autoclaving, allow the mixture to cool down before adding the appropriate antibiotic.
- Pour appropriately 25 ml of the mixture into petri dishes.
Note: Aseptic technique must be employed after the autoclave part.
Go back to our Protocol List
   
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