Revision as of 14:10, 26 October 2010

Parts

We assembled a lot of miBricks (i. e. basic parts) to composite parts that were then used to built up our final constructs. The submitted parts are listed below. We did not categorize the parts according to different subprojects since they are universally applicable or clearly denoted through their names. Some binding site patterns, which were created via [... miraPCR], are listed separately.

basic parts

**table 1**: Our miBricks. Listed ID refers to internal nomenclature.
ID	Content	Registry link	Comment
F1	RSV fw	[http://partsregistry.org/Part:BBa_K337012 BBa_K337012]	-
F2	SV40 rc	[http://partsregistry.org/Part:BBa_K337013 BBa_K337013]	-
F3	RSV rc	[http://partsregistry.org/Part:BBa_K337014 BBa_K337014]	-
F4	BGH fw	[http://partsregistry.org/Part:BBa_K337001 BBa_K337001]	-
F5	BGH rc	[http://partsregistry.org/Part:BBa_K337002 BBa_K337002]	purpose: insertion of BamHI sites
F7	microRNA 10HD	[http://partsregistry.org/Part:BBa_K337016 BBa_K337016]	template for creation of shRNA-like miRNA
F8	CMV fw	[http://partsregistry.org/Part:BBa_K337018 BBa_K337018]	-
F9	FRT site	[http://partsregistry.org/Part:BBa_K337019 BBa_K337019]	-
F10	hRluc	[http://partsregistry.org/Part:BBa_K337025 BBa_K337025]	-
F16	TetR	[http://partsregistry.org/Part:BBa_K337028 BBa_K337028]	-
F17	Luc2	[http://partsregistry.org/Part:BBa_K337030 BBa_K337030]	compatible for insertion of microRNA binding sites
biCMV	biCMV	[http://partsregistry.org/Part:BBa_K337030 BBa_K337017]	-

composite parts

**table 2**: Our composite parts from subsequent assembling steps. Listed ID refers to internal nomenclature.
ID	Content	Registry link	Purpose of the cassette
F6	luc2_sv40ter fw	[http://partsregistry.org/Part:BBa_K337015 BBa_K337015]	Luc2 reporter gene with terminator referring to SV40 promoter
F15	CMV_TetO2	[http://partsregistry.org/Part:BBa_K337027 BBa_K337027]	CMV promoter under control of Tet Operator for regulation of gene expression

final constructs

**table 3**: Our final constructs. Listed ID refers to internal nomenclature.
ID	Content	Registry link	Purpose
K1	pSMB_miTuner Plasmid HD1 (BGH(rc)/shRNA10(rc)/sv40(rc)/RSV/Luc2_sv40/CMV/Kozag_hRluc_BGH )	[http://partsregistry.org/Part:BBa_K337032 BBa_K337032]	gene expression tuning
F15	CMV_TetO2	[http://partsregistry.org/Part:BBa_K337027 BBa_K337027]	CMV promoter under control of Tet Operator for regulation of gene expression

binding site patterns

**table 4**: Binding site patterns generated by miraPCR.
ID	Content	Registry link
1.3	hsa-miR-122 Binding site pattern (3BS)	[http://partsregistry.org/Part:BBa_K337000 BBa_K337000]
1A	hsa-miR-122 Binding site pattern (4BS)	[http://partsregistry.org/Part:BBa_K337003 BBa_K337003]
1-5	hsa-miR-122 Binding site pattern (2BS)	[http://partsregistry.org/Part:BBa_K337004 BBa_K337004]
3.7	hsa-miR-122 Binding site pattern (2BS - additional 10bp Spacer)	[http://partsregistry.org/Part:BBa_K337005 BBa_K337005]
1.5	hsa-miR-122 Binding site pattern (2BS) with randomized nt9-12	[http://partsregistry.org/Part:BBa_K337006 BBa_K337006]
1.8	hsa-miR-122 Binding site pattern (2BS) with randomized nt9-12	[http://partsregistry.org/Part:BBa_K337007 BBa_K337007]
3.1	hsa-miR-122 Binding site pattern (2BS) with randomized nt9-12	[http://partsregistry.org/Part:BBa_K337008 BBa_K337008]
4.5	hsa-miR-122 Binding site pattern (2BS) with randomized nt9-12	[http://partsregistry.org/Part:BBa_K337009 BBa_K337009]
4.6	hsa-miR-122 Binding site pattern (2BS) with randomized nt9-12	[http://partsregistry.org/Part:BBa_K337010 BBa_K337010]
mir221-10L2	hsa-miR-221 Binding site pattern (2BS)	[http://partsregistry.org/Part:BBa_K337010 BBa_K337010]

<groupparts>iGEM010 Heidelberg</groupparts>

@@ Line 105: / Line 105: @@
 |-bgcolor=#cccccc
 |+ align="top, left"|'''table 3''': Our final constructs. Listed ID refers to internal nomenclature.
-|width=40px|  ID||width=300px|Content||width=100px|Registry link||width=450px|Purpose of the cassette
+|width=40px|  ID||width=450px|Content||width=100px|Registry link||width=250px|Purpose
 |-
 |K1||pSMB_miTuner Plasmid HD1 (BGH(rc)/shRNA10(rc)/sv40(rc)/RSV/Luc2_sv40/CMV/Kozag_hRluc_BGH )||[http://partsregistry.org/Part:BBa_K337032 BBa_K337032]|| gene expression tuning

Team:Heidelberg/Parts

From 2010.igem.org