Team:Heidelberg/Parts

From 2010.igem.org

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|+ align="top, left"|'''table 1''': Our miBricks. <br/> Listed ID refers to internal nomenclature.
|+ align="top, left"|'''table 1''': Our miBricks. <br/> Listed ID refers to internal nomenclature.
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|width=40px|  ID||width=100px|Content||width=100px|Registry link|| width=350px|Comment
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|width=40px|  ID||width=100px|Content||width=100px|Registry link|| width=300px|Comment
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|F1||RSV fw||[http://partsregistry.org/Part:BBa_K337012 BBa_K337012]||
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|F1||RSV fw||[http://partsregistry.org/Part:BBa_K337012 BBa_K337012]||-
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|F2||SV40 rc||[http://partsregistry.org/Part:BBa_K337013 BBa_K337013]||
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|F2||SV40 rc||[http://partsregistry.org/Part:BBa_K337013 BBa_K337013]||-
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|F3||RSV rc||[http://partsregistry.org/Part:BBa_K337014 BBa_K337014]||
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|F3||RSV rc||[http://partsregistry.org/Part:BBa_K337014 BBa_K337014]||-
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|F4||BGH fw||[http://partsregistry.org/Part:BBa_K337001 BBa_K337001]||
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|F4||BGH fw||[http://partsregistry.org/Part:BBa_K337001 BBa_K337001]||-
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|F5||BGH rc||[http://partsregistry.org/Part:BBa_K337002 BBa_K337002]|| for insertion of BamHi sites
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|F5||BGH rc||[http://partsregistry.org/Part:BBa_K337002 BBa_K337002]|| for insertion of BamHI sites
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|F7||microRNA 10HD||[http://partsregistry.org/Part:BBa_K337016 BBa_K337016]||
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|F7||microRNA 10HD||[http://partsregistry.org/Part:BBa_K337016 BBa_K337016]||-
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|F8||CMV fw||[http://partsregistry.org/Part:BBa_K337018 BBa_K337018]||
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|F8||CMV fw||[http://partsregistry.org/Part:BBa_K337018 BBa_K337018]||-
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|F9||FRT site||[http://partsregistry.org/Part:BBa_K337019 BBa_K337019]||
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|F9||FRT site||[http://partsregistry.org/Part:BBa_K337019 BBa_K337019]||-
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|F10||hRluc||[http://partsregistry.org/Part:BBa_K337025 BBa_K337025]||
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|F10||hRluc||[http://partsregistry.org/Part:BBa_K337025 BBa_K337025]||-
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|F16||TetR||[http://partsregistry.org/Part:BBa_K337028 BBa_K337028]||
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|F16||TetR||[http://partsregistry.org/Part:BBa_K337028 BBa_K337028]||-
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|F17||Luc2*||[http://partsregistry.org/Part:BBa_K337030 BBa_K337030]|| compatible for insertion of microRNA binding sites
|F17||Luc2*||[http://partsregistry.org/Part:BBa_K337030 BBa_K337030]|| compatible for insertion of microRNA binding sites
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|width=40px|  ID||width=300px|Content||width=100px|Registry link||width=450px|Purpose of the cassette  
|width=40px|  ID||width=300px|Content||width=100px|Registry link||width=450px|Purpose of the cassette  
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|F 6||luc2_sv40ter fw||[http://partsregistry.org/Part:BBa_K337015 BBa_K337015]|| Luc2 reporter gene with terminator referring to SV40 promoter  
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|F6||luc2_sv40ter fw||[http://partsregistry.org/Part:BBa_K337015 BBa_K337015]|| Luc2 reporter gene with terminator referring to SV40 promoter  
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|F 15||CMV_TetO2||[http://partsregistry.org/Part:BBa_K337027 BBa_K337027]|| CMV promoter under control of Tet Operator for regulation of gene expression
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|F15||CMV_TetO2||[http://partsregistry.org/Part:BBa_K337027 BBa_K337027]|| CMV promoter under control of Tet Operator for regulation of gene expression
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Revision as of 11:27, 26 October 2010


Parts

We assembled a lot of miBricks (i. e. basic parts) to composite parts that were then used to built up our final constructs. The submitted parts are listed below. We did not categorize the parts according to different subprojects since they are universally applicable or clearly denoted through their names.

basic parts

table 1: Our miBricks.
Listed ID refers to internal nomenclature.
IDContentRegistry linkComment
F1RSV fw[http://partsregistry.org/Part:BBa_K337012 BBa_K337012]-
F2SV40 rc[http://partsregistry.org/Part:BBa_K337013 BBa_K337013]-
F3RSV rc[http://partsregistry.org/Part:BBa_K337014 BBa_K337014]-
F4BGH fw[http://partsregistry.org/Part:BBa_K337001 BBa_K337001]-
F5BGH rc[http://partsregistry.org/Part:BBa_K337002 BBa_K337002] for insertion of BamHI sites
F7microRNA 10HD[http://partsregistry.org/Part:BBa_K337016 BBa_K337016]-
F8CMV fw[http://partsregistry.org/Part:BBa_K337018 BBa_K337018]-
F9FRT site[http://partsregistry.org/Part:BBa_K337019 BBa_K337019]-
F10hRluc[http://partsregistry.org/Part:BBa_K337025 BBa_K337025]-
F16TetR[http://partsregistry.org/Part:BBa_K337028 BBa_K337028]-
F17Luc2*[http://partsregistry.org/Part:BBa_K337030 BBa_K337030] compatible for insertion of microRNA binding sites

composite parts

table 2: Our composite parts from subsequent assembling steps. Listed ID refers to internal nomenclature.
IDContentRegistry linkPurpose of the cassette
F6luc2_sv40ter fw[http://partsregistry.org/Part:BBa_K337015 BBa_K337015] Luc2 reporter gene with terminator referring to SV40 promoter
F15CMV_TetO2[http://partsregistry.org/Part:BBa_K337027 BBa_K337027] CMV promoter under control of Tet Operator for regulation of gene expression

<groupparts>iGEM010 Heidelberg</groupparts>



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