Team:Stockholm/13 October 2010

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{{Stockholm/Top2}}
{{Stockholm/Top2}}
==Andreas==
==Andreas==
 +
===Plasmid prep===
 +
''From 12/10 ON cultures''
 +
 +
{|border="1" cellpadding="1" cellspacing="0"
 +
!colspan="3"|DNA concentration
 +
|-
 +
!Sample
 +
!width="60"|Conc [ng/μl]
 +
!width="60"|A<sub>260</sub>/A<sub>280</sub>
 +
|-
 +
|pSB1C3.IgGp
 +
|align="center"|237.9
 +
|align="center"|1.89
 +
|-
 +
|pSB1C3.bFGF
 +
|align="center"|210.3
 +
|align="center"|1.91
 +
|-
 +
|pSB1C3.ProtA
 +
|align="center"|173.6
 +
|align="center"|1.89
 +
|-
 +
|pSB1C3.yCCS
 +
|align="center"|229.2
 +
|align="center"|1.91
 +
|-
 +
|pSB1C3.SOD
 +
|align="center"|193.0
 +
|align="center"|1.87
 +
|}
 +
 +
===Sequencing===
 +
*15 &mu;l plasmid DNA; 1.5 &mu;l primer (VR)
 +
 +
{|border="1" cellpadding="1" cellspacing="0"
 +
!colspan="3"|DNA concentration
 +
|-
 +
!Sample
 +
!width="120"|Label
 +
!width="120"|Sequence code
 +
|-
 +
|pSB1C3.IgGp
 +
|align="center"|pSB1C3.IgGp_VR
 +
|align="center"|ASB0045 776
 +
|-
 +
|pSB1C3.bFGF
 +
|align="center"|pSB1C3.bFGF_VR
 +
|align="center"|ASB0045 777
 +
|-
 +
|pSB1C3.ProtA
 +
|align="center"|pSB1C3.ProtA_VR
 +
|align="center"|ASB0045 778
 +
|-
 +
|pSB1C3.yCCS
 +
|align="center"|pSB1C3.yCCS_VR
 +
|align="center"|ASB0045 779
 +
|-
 +
|pSB1C3.SOD
 +
|align="center"|pSB1C3.SOD_VR
 +
|align="center"|ASB0045 780
 +
|}
 +
 +
----
 +
==Nina==
 +
 +
===concentration measurement===
 +
 +
I measured the concentration of the gel cleaned up samples from yesterday by spectrophotometry.
 +
 +
[[Image:Aq16.jpg]]
 +
 +
===Ligation===
 +
 +
I ligated the gel cleaned samples.
 +
 +
[[Image:Aq17.jpg]]
 +
 +
===Transformation===
 +
 +
I transformed the ligation samples into nine BL21 tubes each with 100 ul cells. I added 6 ul of the ligations to the cells and I thawed the tubes in the first step in 15 min.
 +
 +
I hope this will save some time since I am skipping the transformation into Top 10 cells. We don't have much time left of the competition therefore I hope this will work.
 +
 +
 +
 +
 +
== Mimmi ==
 +
 +
=== Home page design ===
 +
 +
*Bacteria figures for each link...
 +
**saftey
 +
**Sponsors
 +
**Project
 +
**Planning
 +
**News
 +
**Team
 +
**Modelling
 +
**Notebook
 +
**Protocols
 +
 +
*to do (?):
 +
**Results
 +
**BioBricks
 +
**Assemblies
 +
 +
 +
 +
=== SOD - growth curve ===
 +
- ON culture
 +
 +
*SOD.his
 +
*LMWP.SOD.his
 +
*TAT.SOD.his
 +
*Tra10.SOD.his
 +
 +
 +
*3ml Amp<sub>100</sub> + toothpick of glycerol stock
 +
 +
{{Stockholm/Footer}}

Latest revision as of 11:10, 26 October 2010

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Contents

Andreas

Plasmid prep

From 12/10 ON cultures

DNA concentration
Sample Conc [ng/μl] A260/A280
pSB1C3.IgGp 237.9 1.89
pSB1C3.bFGF 210.3 1.91
pSB1C3.ProtA 173.6 1.89
pSB1C3.yCCS 229.2 1.91
pSB1C3.SOD 193.0 1.87

Sequencing

  • 15 μl plasmid DNA; 1.5 μl primer (VR)
DNA concentration
Sample Label Sequence code
pSB1C3.IgGp pSB1C3.IgGp_VR ASB0045 776
pSB1C3.bFGF pSB1C3.bFGF_VR ASB0045 777
pSB1C3.ProtA pSB1C3.ProtA_VR ASB0045 778
pSB1C3.yCCS pSB1C3.yCCS_VR ASB0045 779
pSB1C3.SOD pSB1C3.SOD_VR ASB0045 780

Nina

concentration measurement

I measured the concentration of the gel cleaned up samples from yesterday by spectrophotometry.

Aq16.jpg

Ligation

I ligated the gel cleaned samples.

Aq17.jpg

Transformation

I transformed the ligation samples into nine BL21 tubes each with 100 ul cells. I added 6 ul of the ligations to the cells and I thawed the tubes in the first step in 15 min.

I hope this will save some time since I am skipping the transformation into Top 10 cells. We don't have much time left of the competition therefore I hope this will work.



Mimmi

Home page design

  • Bacteria figures for each link...
    • saftey
    • Sponsors
    • Project
    • Planning
    • News
    • Team
    • Modelling
    • Notebook
    • Protocols
  • to do (?):
    • Results
    • BioBricks
    • Assemblies


SOD - growth curve

- ON culture

  • SOD.his
  • LMWP.SOD.his
  • TAT.SOD.his
  • Tra10.SOD.his


  • 3ml Amp100 + toothpick of glycerol stock





The Faculty of Science at Stockholm University Swedish Vitiligo association (Svenska Vitiligoförbundet) Geneious Fermentas/ Sigma-Aldrich/

Retrieved from "http://2010.igem.org/Team:Stockholm/13_October_2010"