"
Team:Chiba/Notebook 3
From 2010.igem.org
(Difference between revisions)
| Line 257: | Line 257: | ||
Double transformation | Double transformation | ||
| - | - | + | Strain:XL10-Gold |
| - | Plux inv-GFP | + | Plasmid:Plux inv-GFP & Ptrc-LuxR |
| - | Ptrc-LuxR | + | |
| - | + | ↓ | |
| + | not Fuluorescence | ||
</pre> | </pre> | ||
| + | <pre> | ||
| + | Double transformation | ||
| + | |||
| + | Strain:DH10B | ||
| + | Plasmid:Plux inv-GFP & Plac-LuxR | ||
Revision as of 08:03, 26 October 2010
Lux inverter
2010-10-8
transformation BBa_R0061 BBa_E0240
2010-10-9
Mini-prep BBa_R0061 BBa_E0240 digestion -------------------- BBa_R0061 20 µL 10×Buffer 3 µL SpeI 1 µL PstI 1 µL NFW 5 µL -------------------- -------------------- BBa_E0240 20 µL 10×Buffer 3 µL XbaI 1 µL PstI 1 µL NFW 5 µL -------------------- ↓ gel extraction
2010-10-11
Ligation ----------------------- BBa_R0061 (70 ng) 1 µL BBa_E0240 (100 ng) 2 µL ATP (10 mM) 1 µL 10×Buffer 1 µL T4 DNA Ligase 1 µL NFW 4 µL ----------------------- ↓ transformation
2010-10-12
Ligation ----------------------- BBa_R0061(100 ng) 1 µL BBa_E0240 (100 ng) 3 µL ATP (10 mM) 1 µL T4 DNA Ligase 1 µL dH<sub>2</sub>O 4 µL ----------------------- ↓ transformation
2010-10-13
Double transformation Strain:XL10-Gold Plasmid:Plux inv-GFP & Ptrc-LuxR ↓ not Fuluorescence
Double transformation Strain:DH10B Plasmid:Plux inv-GFP & Plac-LuxR
