Team:Chiba/Notebook 3

From 2010.igem.org

(Difference between revisions)
Line 196: Line 196:
<html><h3>2010-10-9</h3></html>
<html><h3>2010-10-9</h3></html>
-
Mini-prep
+
 
 +
<pre>Mini-prep
 +
 
BBa_R0061
BBa_R0061
BBa_E0240
BBa_E0240
digestion
digestion
 +
--------------------
BBa_R0061    20 µL
BBa_R0061    20 µL
10×Buffer    3 µL
10×Buffer    3 µL
SpeI　　　　　 1 µL
SpeI　　　　　 1 µL
PstI　　　　　 1 µL
PstI　　　　　 1 µL
-
dH<sub>2</sub>O5 µL
+
NFW            5 µL
--------------------
--------------------
Line 213: Line 216:
XbaI            1 µL
XbaI            1 µL
PstI　　　　　  1 µL
PstI　　　　　  1 µL
-
dH<sub>2</sub>O 5 µL
+
NFW            5 µL
--------------------
--------------------
-
 
+
↓
gel extraction
gel extraction
-
<br>
+
</pre>
 +
 
<html><h3>2010-10-11</h3></html>
<html><h3>2010-10-11</h3></html>
<pre>
<pre>
Ligation
Ligation
-
 
+
-----------------------
BBa_R0061 (70 ng)  1 µL       
BBa_R0061 (70 ng)  1 µL       
BBa_E0240 (100 ng) 2 µL
BBa_E0240 (100 ng) 2 µL
Line 229: Line 233:
10×Buffer        1 µL
10×Buffer        1 µL
T4 DNA Ligase      1 µL
T4 DNA Ligase      1 µL
-
dH<sub>2</sub>O    4 µL
+
NFW                4 µL
-
 
+
-----------------------
 +
↓
transformation
transformation
</pre>
</pre>
 +
<html><h3>2010-10-12</h3></html>
<html><h3>2010-10-12</h3></html>
<pre>Ligation
<pre>Ligation
-
 
+
-----------------------
BBa_R0061(100 ng)  1 µL
BBa_R0061(100 ng)  1 µL
BBa_E0240 (100 ng) 3 µL
BBa_E0240 (100 ng) 3 µL
Line 242: Line 248:
T4 DNA Ligase      1 µL
T4 DNA Ligase      1 µL
dH<sub>2</sub>O    4 µL
dH<sub>2</sub>O    4 µL
-
 
+
-----------------------
-
<html><h3>2010-10-13</h3></html></pre>
+
↓
 +
transformation
 +
</pre>
 +
<html><h3>2010-10-13</h3></html>

Revision as of 07:04, 26 October 2010




 


Lux inverter

2010-10-8


transformation

BBa_R0061
BBa_E0240


2010-10-9

Mini-prep

BBa_R0061
BBa_E0240

digestion
--------------------
BBa_R0061     20 µL
10×Buffer     3 µL
SpeI　　　　　 1 µL
PstI　　　　　 1 µL
NFW            5 µL
--------------------

--------------------
BBa_E0240      20 µL
10×Buffer      3 µL
XbaI            1 µL
PstI　　　　　  1 µL
NFW             5 µL
--------------------
↓
gel extraction


2010-10-11

Ligation
-----------------------
BBa_R0061 (70 ng)  1 µL       
BBa_E0240 (100 ng) 2 µL
ATP (10 mM)        1 µL
10×Buffer         1 µL
T4 DNA Ligase      1 µL
NFW                4 µL
-----------------------
↓
transformation

2010-10-12

Ligation
-----------------------
BBa_R0061(100 ng)  1 µL
BBa_E0240 (100 ng) 3 µL
ATP (10 mM)        1 µL
T4 DNA Ligase      1 µL
dH<sub>2</sub>O    4 µL
-----------------------
↓
transformation

2010-10-13



　

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