Team:Heidelberg/Notebook/Methods
From 2010.igem.org
(→Dual Luciferase Assay) |
(→Transfection) |
||
Line 63: | Line 63: | ||
DAY2 | DAY2 | ||
*transfection | *transfection | ||
- | **to media (OptiMEM, no FBS) add FuGENE reagent (it is important to add FuGENE to media, not other way round and not to drop FuGENE on the walls of the tube) | + | **to media (OptiMEM, no FBS) add [http://www.roche-applied-science.com/pack-insert/1815091a.pdf FuGENE 6 reagent] (it is important to add FuGENE to media, not other way round and not to drop FuGENE on the walls of the tube) |
**incubate 5 minutes at room temperature | **incubate 5 minutes at room temperature | ||
**add DNA | **add DNA |
Revision as of 06:23, 26 October 2010
|
|
|||||||||||||||||||||||||||||||||