Team:Brown/Project

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Revision as of 05:47, 26 October 2010

Project 1 - Light-Pattern Controlled Circuit

Project Description

The aim of this project is to create a cell that can go through a sequence of four distinct states in response to a timed pattern of light-on, light-off.

This project makes use of parts from EPF Lusanne '09 (the LOVTAP light activated promoter), PKU '07/'09 (bistable switch, AND gate), and Missouri Western State University '08 (Hybrid promoter). Our goal is to show that it is possible and easy to create entirely new cell logic systems from parts that already exist in the registry.

See the model for this system here:Team:Brown/Modeling

Project 2 - TAT-PTD

E.Cargo

We designed a modular Tat-Linker Biobrick that will allow for easy fusion of the Tat-PTD to any other biobricked proteins. Specifically, we aimed to fuse this Tat-Linker in RFC25 format to two bacterial transcription factors, LacI and AraC, with the intent of using the two Tat-TFs as a tool to induce transient gene expression in E. coli without the need to follow through with more time-consuming cell transformation protocols. We saw a potential application in the portion of the Light-Pattern Controlled Circuit of our project, as well as in any other future genetic circuits that require part-by-part testing.

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 == '''Project 2 - TAT-PTD''' ==
-=== Project Description ===
+=== E.Cargo ===
-We have created a recombinant protein which fuses a Tat-protein transduction domain (Tat-ptd) with a synthetic single chain variable fragment (scFv) domain. The goals of this project are: 1) be able to stably express the fusion protein in E. Coli; 2) create a protein that will retain conformation and function in cytosolic conditions; 3) the intrabody should be able to bind with high specificity to the desired target protein; 4) the intrabody can translocate across mammalian cell and tissue barriers; and 5) include a method for easy fluorescence reporting.
+We designed a modular Tat-Linker Biobrick that will allow for easy fusion of the Tat-PTD to any other biobricked proteins. Specifically, we aimed to fuse this Tat-Linker in RFC25 format to two bacterial transcription factors, LacI and AraC, with the intent of using the two Tat-TFs as a tool to induce transient gene expression in <i>E. coli</i> without the need to follow through with more time-consuming cell transformation protocols. We saw a potential application in the portion of the Light-Pattern Controlled Circuit of our project, as well as in any other future genetic circuits that require part-by-part testing.
-We also are in the process of creating a biobrick that will allow for easy fusion of the Tat-PTD to another biobricked proteins. We plan on characterizing this biobrick by combining it with transcription factors in the Registry and  transiently acting on inducible/repressible reporter constructs.