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Team:Newcastle/10 September 2010
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| + | =Characterization of ''yneA''= | ||
| + | |||
| + | ==Aim== | ||
| + | |||
| + | The aim of this experiment is to prepare slides for the ''Bacillus subtilis'' 168 cells which have ''yneA'' on the plasmid (pMutin4 and pMap65) and under ''lacI'' regulation and are induced by IPTG at various concentrations. | ||
| + | |||
| + | ==Materials and Protocol== | ||
| + | |||
| + | Please refer to: [[Team:Newcastle/IPTG INduction|Slide preparation for IPTG induced cells]] for materials and protocol. | ||
| + | For this experiment we used the following cell colonies: | ||
| + | #''Bacillus subtilis'' 168 | ||
| + | #''Bacillus subtilis'' 168 with pMutin4 having ''yneA'' insert | ||
| + | #''Bacillus subtilis'' 168 tith pMap65 having ''yneA'' insert | ||
| + | Grow them overnight. | ||
| + | |||
| + | ==Result and Conclusion== | ||
| + | Please refer to: [[Team:Newcastle/10_September_2010|10.10.2010]] for result and conclusion of the experiment. | ||
{{Team:Newcastle/footer}} | {{Team:Newcastle/footer}} | ||
Revision as of 02:59, 26 October 2010
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Contents |
Characterization of yneA
Aim
The aim of this experiment is to prepare slides for the Bacillus subtilis 168 cells which have yneA on the plasmid (pMutin4 and pMap65) and under lacI regulation and are induced by IPTG at various concentrations.
Materials and Protocol
Please refer to: Slide preparation for IPTG induced cells for materials and protocol. For this experiment we used the following cell colonies:
- Bacillus subtilis 168
- Bacillus subtilis 168 with pMutin4 having yneA insert
- Bacillus subtilis 168 tith pMap65 having yneA insert
Grow them overnight.
Result and Conclusion
Please refer to: 10.10.2010 for result and conclusion of the experiment.
   
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