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Team:Brown/Protocols
From 2010.igem.org
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PCR Cleanup/Purification | PCR Cleanup/Purification | ||
*[[Media:QIAquick_Spin_Handbook.pdf|PCR Cleanup/Purification PDF (page 19)]] | *[[Media:QIAquick_Spin_Handbook.pdf|PCR Cleanup/Purification PDF (page 19)]] | ||
| + | Gel Electrophoresis | ||
| + | *[[:Team:Brown/Protocols/Gels|Agarose gels]] | ||
Gel Extraction | Gel Extraction | ||
*[[Media:QIAquick_Spin_Handbook.pdf|Gel Extraction PDF (page 25)]] | *[[Media:QIAquick_Spin_Handbook.pdf|Gel Extraction PDF (page 25)]] | ||
Revision as of 01:48, 26 October 2010
Protocols
Here are the protocols that we followed. Occasionally we make exceptions to the general "recipe," but those exceptions are documented in our Notebook.
PCR
PCR Cleanup/Purification
Gel Electrophoresis
Gel Extraction
Ligations
Restriction digests
Overnights
Transformations
Cell competence protocols
Minipreps
