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Protocol/7
From 2010.igem.org
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Protocol 7: Gel Extraction | Protocol 7: Gel Extraction | ||
| - | + | ||
| + | Reagents: | ||
* Clean scalpel | * Clean scalpel | ||
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* Eppendorf tube | * Eppendorf tube | ||
* Gel extraction kit | * Gel extraction kit | ||
| - | * | + | * 55<sup>o</sup>C water bath |
* Vortex | * Vortex | ||
* Microfuge | * Microfuge | ||
| - | Procedure | + | Procedure: |
* Place the gel on the transilluminator. Put on face shield and gloves. | * Place the gel on the transilluminator. Put on face shield and gloves. | ||
| Line 30: | Line 31: | ||
* Pry out the gel ‘cube’ and place it in an eppendorf tube. | * Pry out the gel ‘cube’ and place it in an eppendorf tube. | ||
* Follow instructions on pp. 25 of [http://www.qiagen.com/literature/render.aspx?id=103715 QIAQuick Spin Handbook]. | * Follow instructions on pp. 25 of [http://www.qiagen.com/literature/render.aspx?id=103715 QIAQuick Spin Handbook]. | ||
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[[Team:Alberta/Notebook/protocols| Back]] | [[Team:Alberta/Notebook/protocols| Back]] | ||
{{Team:Alberta/endMainContent}} | {{Team:Alberta/endMainContent}} | ||
Revision as of 01:18, 26 October 2010
Protocol 7: Gel Extraction
Reagents:
- Clean scalpel
- Transilluminator
- Eppendorf tube
- Gel extraction kit
- 55oC water bath
- Vortex
- Microfuge
Procedure:
- Place the gel on the transilluminator. Put on face shield and gloves.
- Turn on transilluminator and quickly make four slices; one on each side of the band you want to cut out and turn transilluminator off. Keep exposure to a minimum.
- Pry out the gel ‘cube’ and place it in an eppendorf tube.
- Follow instructions on pp. 25 of [http://www.qiagen.com/literature/render.aspx?id=103715 QIAQuick Spin Handbook].
