Protocol/3
From 2010.igem.org
(Difference between revisions)
Line 26: | Line 26: | ||
* Add 350uL of overnight culture to each tube. | * Add 350uL of overnight culture to each tube. | ||
* Pipet up and down to gently mix. | * Pipet up and down to gently mix. | ||
- | * Flash freeze in liquid N2 or dry ice/methanol bath | + | * Flash freeze in liquid N2 or dry ice/methanol bath. |
* Place in -80<sup>o</sup>C freezer when frozen. | * Place in -80<sup>o</sup>C freezer when frozen. | ||
+ | |||
Notes: | Notes: |
Revision as of 01:02, 26 October 2010
Protocol 3: Glycerol Stock
Reagents:
- Overnight with bacterial growth (probably 1.5mL)
- Screw cap tubes
- Glycerol
Procedure:
- Pipet 150uL 50% glycerol into 3* properly labeled 1.5 ml screw cap tubes.
- Add 350uL of overnight culture to each tube.
- Pipet up and down to gently mix.
- Flash freeze in liquid N2 or dry ice/methanol bath.
- Place in -80oC freezer when frozen.
Notes:
- If we make extra we have them if we need to send them out or want to store in several locations.