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Protocol/3
From 2010.igem.org
(Difference between revisions)
| Line 14: | Line 14: | ||
| - | + | Reagents: | |
| - | * Overnight with bacterial growth (probably 1. | + | * Overnight with bacterial growth (probably 1.5mL) |
* Screw cap tubes | * Screw cap tubes | ||
* Glycerol | * Glycerol | ||
| Line 23: | Line 23: | ||
Procedure: | Procedure: | ||
| - | + | * Pipet 150uL 50% glycerol into 3* properly labeled 1.5 ml screw cap tubes. | |
| - | * Pipet | + | * Add 350uL of overnight culture to each tube. |
| - | * Add | + | |
* Pipet up and down to gently mix. | * Pipet up and down to gently mix. | ||
* Flash freeze in liquid N2 or dry ice/methanol bath | * Flash freeze in liquid N2 or dry ice/methanol bath | ||
| - | * Place in -80 C freezer when frozen. | + | * Place in -80<sup>o</sup>C freezer when frozen. |
| + | |||
| + | Notes: | ||
| - | + | * If we make extra we have them if we need to send them out or want to store in several locations. | |
| - | |||
[[Team:Alberta/Notebook/protocols| Back]] | [[Team:Alberta/Notebook/protocols| Back]] | ||
{{Team:Alberta/endMainContent}} | {{Team:Alberta/endMainContent}} | ||
Revision as of 01:02, 26 October 2010
Protocol 3: Glycerol Stock
Reagents:
- Overnight with bacterial growth (probably 1.5mL)
- Screw cap tubes
- Glycerol
Procedure:
- Pipet 150uL 50% glycerol into 3* properly labeled 1.5 ml screw cap tubes.
- Add 350uL of overnight culture to each tube.
- Pipet up and down to gently mix.
- Flash freeze in liquid N2 or dry ice/methanol bath
- Place in -80oC freezer when frozen.
Notes:
- If we make extra we have them if we need to send them out or want to store in several locations.
