Team:Newcastle/Meetings/26 March 2010

From 2010.igem.org

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Mutations- How stop general questions  
Mutations- How stop general questions  
Not specifically a problem – keep thinking about it though  
Not specifically a problem – keep thinking about it though  
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  |5. Action points
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====Action points====
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*All - Read Voygt paper  
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|All -Read Voygt paper  
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*All - Next week working off the proper wiki
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*Janetta contact details doc in drop box  
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|All-Next week working off the proper wiki!!!
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*Janetta and Richard – Flow chart!!!  
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*Reminder to neil and jen not welcome trust funding by 2nd of April?  
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|Janetta contact details doc in drop box  
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*Janetta- T-shirt sponsors and logo – embroidered / print mixed – 5 or 6 sponsors design your own- printing cheaper , quotes dependent on design  
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|-
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*Jannetta- send us links for graphic design software- please
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|Janetta and Richard – Flow chart!!!  
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*Transfer the wiki  
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|-
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*Individual iGEM applications - Phil hasn’t applied; Richard not accepted; Janetta text Phil
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|Reminder to neil and jen not welcome trust funding by 2nd of April?  
+
*Jen/Neil - Pay for team!!
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|-
+
*Visas/Passport update - Harsh will apply May/June; Richard needs to get a visa waiver  
-
|Janetta- T-shirt sponsors and logo – embroidered / print mixed – 5 or 6 sponsors design your own- printing cheaper , quotes dependent on design  
+
====Wiki transfer====
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|-
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*Clarify contents of identified pages
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|Janetta- send us links for graphic design software- please
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*Ethics page
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|-
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*Research page  
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|Transfer the wiki  
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*Safety page  
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|-
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*Identify other pages required
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    |1. Individual iGEM applications
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*Ideas for layout/theme
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|-
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*Zoltan Tidy it up-Urgent  
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|Phil hasn’t applied  
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*Borrow last years / Look at anything thats nice  
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|-
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*It evolves!!! We can change it!!! Now!!!!!!!  
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|Richard not accepted  
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====Travel plans====
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|-
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*Flights and Accomodation
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|Janetta text Phil  
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**Need to sort out  who is going  
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|-
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====Funding====
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|Jen/Neil-Pay for team!!
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*Harsh has made draft letter  
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|-
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*Main chemical company – free stuff?
-
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====Advisors/additional team members - civil engineer/graphics designer====
-
 
+
*Still looking – Neil,Jen  
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        |2. Visas/Passport update
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====Tutorials====
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|-
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#Sequentia and Clone manager - basics  
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|Harsh- May June apply
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#Modelling part two  
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|-
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====What next?====
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|Richard visa waiver  
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*Flow chart of what we’re going to do Big picture!!!  
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|-
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*If its coloured life is easier  
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        |3. Wiki transfer
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*Cambridge brick - Colour
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|-
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*Web services for Biosynthetics, Flow chart
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              |1. Clarify contents of identified pages
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*Taverner
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              |2. Id Ethics page  
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|Research page  
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|Safety page  
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|-
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|entify other pages required
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|-
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-
 
+
-
              |3. Ideas for layout / theme
+
-
|-
+
-
|Zoltan Tidy it up-Urgent  
+
-
|-
+
-
|Borrow last years / Look at anything thats nice  
+
-
|-
+
-
|It evolves!!! We can change it!!! Now!!!!!!!  
+
-
|-
+
-
 
+
-
 
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    |4. Travel plans
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              |1. Flights
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|-
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|Need to sort out who is going
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|-
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              |2. Accomodation
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|-
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|Need to sort out  who is going  
+
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|-
+
-
        |5. Funding
+
-
|-
+
-
|Harsh has made draft letter  
+
-
|-
+
-
|Main chemical company – free stuff ?
+
-
 
+
-
|-
+
-
        |6. Advisors/additional team members - civil engineer/graphics designer?
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-
|-
+
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|Still looking –Neil,Jen  
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-
|-
+
-
 
+
-
        |7. Tutorials
+
-
|-
+
-
|Sequentia and Clone manager- basics  
+
-
|-
+
-
|Modelling part two  
+
-
|-
+
-
        |8. What next?
+
-
|-
+
-
|Flow chart of what we’re going to do Big picture!!!  
+
-
|-
+
-
|If its coloured life is easier  
+
-
|-
+
-
|Cambridge brick-Colour
+
-
|-
+
-
|Web services for Biosynthetics, Flow chart
+
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|-
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|Deverner
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====Time line discussion====
====Time line discussion====
*Need someone else on time line.  
*Need someone else on time line.  
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====Other business====
====Other business====
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*Pdf mark up tools – PDF exchange viewer, foxit, keeps mark-up  
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*PDF mark up tools – PDF exchange viewer, foxit, keeps mark-up  
*Making dropbox use better  
*Making dropbox use better  

Revision as of 00:42, 26 October 2010

iGEM Homepage Newcastle University BacillaFilla Homepage Image Map

Contents

Formal meeting - 26th March 2010

  • Room: CBCB; Start: 11 am
  • Chair: Jannetta, Minutes: Rachel, Computer: Harsh
  • Present: Jen, Janetta, Neil, Steven,Younus, Da, Alan, Richard, Harsh
  • Apologies: Phil (Holiday), Jem and Matt
  • Approval of agenda and minutes - Approved

4. Research Feedback

Properties of concrete and surface tension (Zoltan and Steven)

  • High compressive strength low tensile strength
  • Steal added good for temp change
  • CaCO3 – similar to concrete; expand same as concrete... might crack like concrete
  • Spider silk like steal- reduce possibility of cracking
  • Glue- no many papers- expansion may not be a problem?
  • Silk alkali resistant
  • Capillary properties of concrete change over time- in project we consider small rapid
  • Cement water ratio and surface area and environment
  • Where to look for the cracks? Crack pattern

Microinjection (Zoltan and Steven)

  • Simple issue
  • Not correct term
  • Swimming Bacillus
  • Oxygen gradient big selling points

Genetics Homologous recombination plasmid chromosome (Harsh and Rachel)

  • Test in plasmids- Find good plasmid
  • Integrate into genome 
  • Recombination site... Find!!!
  • amyE site amylase with insert can’t break down amylase

Nutrients (Harsh and Rachel)

  • Bacteria algae growth living in concrete
  • Only activate feed bacillus
  • Two hybrid
  • Biofilms exclude other not algae
  • Antifungals, antialgaes, antibiotic

Sporulation, germination and secretion control (Alan and Da)

  • SpoA – last years team sporulation control, maybe turn off completely
  • Nutrient availability
  • KinA
  • Inducible promoter
  • Glue out of bug

Quorum sensing (Younus and Phil)

  • CompX and cff
  • Two-component system comP

Kill switch (Richard and Jannetta)

  • Why ? Don’t want?- not die after job fill new cracks
  • Nuclease
  • Phil masEF
  • Skf SbB
  • If linear DNA works


Glue, CaCo3 and spider silk (Zoltan and Jannetta)

  • Bacteria that produce CaCo3 from urea
  • How secrete spider silk long complex proteins – how can we make it happen in bacillus
  • Secretion systems

Other points

  • Linear DNA – Richard

Justifications of Linear Build it up the use. Mutations- How stop general questions Not specifically a problem – keep thinking about it though

Action points

  • All - Read Voygt paper
  • All - Next week working off the proper wiki
  • Janetta contact details doc in drop box
  • Janetta and Richard – Flow chart!!!
  • Reminder to neil and jen not welcome trust funding by 2nd of April?
  • Janetta- T-shirt sponsors and logo – embroidered / print mixed – 5 or 6 sponsors design your own- printing cheaper , quotes dependent on design
  • Jannetta- send us links for graphic design software- please
  • Transfer the wiki
  • Individual iGEM applications - Phil hasn’t applied; Richard not accepted; Janetta text Phil
  • Jen/Neil - Pay for team!!
  • Visas/Passport update - Harsh will apply May/June; Richard needs to get a visa waiver

Wiki transfer

  • Clarify contents of identified pages
  • Ethics page
  • Research page
  • Safety page
  • Identify other pages required
  • Ideas for layout/theme
  • Zoltan Tidy it up-Urgent
  • Borrow last years / Look at anything thats nice
  • It evolves!!! We can change it!!! Now!!!!!!!

Travel plans

  • Flights and Accomodation
    • Need to sort out who is going

Funding

  • Harsh has made draft letter
  • Main chemical company – free stuff?

Advisors/additional team members - civil engineer/graphics designer

  • Still looking – Neil,Jen

Tutorials

  1. Sequentia and Clone manager - basics
  2. Modelling part two

What next?

  • Flow chart of what we’re going to do Big picture!!!
  • If its coloured life is easier
  • Cambridge brick - Colour
  • Web services for Biosynthetics, Flow chart
  • Taverner

Time line discussion

  • Need someone else on time line.
  • 16th of June Lab induction
  • Deadlines soon
  • Change flight deadline – not 31st

Other business

  • PDF mark up tools – PDF exchange viewer, foxit, keeps mark-up
  • Making dropbox use better

Items for next agenda

  • Need wiki transferred by next meeting
  • Review of flow chart if done

Next meeting

  • Thursday early as possible – email to organise
  • Chair: Da, Minutes: Alan, Computer: Younus