Team:Newcastle/11 August 2010
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+ | =Gel electrophoresis for the subtilin immunity fragments= | ||
+ | |||
+ | ==Aims== | ||
+ | |||
+ | The aim of the experiment is to check whether we have the correct fragment sizes on the four subtilin fragments that are we have amplified [[Team:Newcastle/10 August 2010#Subtilin_Immunity_BioBrick| yesterday]]. | ||
+ | |||
+ | ==Materials and protocol== | ||
+ | |||
+ | Please refer to the [[Team:Newcastle/Gel_electrophoresis| gel electrophoresis]] protocol. | ||
==Results== | ==Results== | ||
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- | * '''Lane 1''': | + | [[Image:Newcastle Subtilin Immunity Gel 1b.jpg|300px]] |
- | * '''Lane 2''': Plasmid Vector | + | |
+ | '''Figure 1''': Gel electrophoresis of the PCR products of the parts required for the subtilin immunity BioBrick. | ||
+ | * '''Lane 1''': 1 kb DNA ladder | ||
+ | * '''Lane 2''': Plasmid Vector (pSB1C3) | ||
* '''Lane 3''': Promoter and RBS (pVeg-SpoVG) | * '''Lane 3''': Promoter and RBS (pVeg-SpoVG) | ||
* '''Lane 4''': ''spaIFEG'' Gene Cluster | * '''Lane 4''': ''spaIFEG'' Gene Cluster | ||
* '''Lane 5''': Double terminator | * '''Lane 5''': Double terminator | ||
- | * '''Lane 6''': | + | * '''Lane 6''': 100 bp DNA ladder |
+ | |||
+ | ==Discussion== | ||
+ | The correct band size were observed for for the Plasmid Vector (lane 2), Promoter & RBS (lane 3) and Double terminator (lane 5). However no band was observed for the ''spaIFEG''(lane 4). | ||
+ | ==Conclusion== | ||
+ | No band was observed for the ''spaIFEG''. This could be due to the wrong melting temperature. | ||
+ | '''Go back to our main [[Team:Newcastle/notebook| Lab book]] page''' | ||
{{Team:Newcastle/footer}} | {{Team:Newcastle/footer}} |
Latest revision as of 00:11, 26 October 2010
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Contents |
Gel electrophoresis for the subtilin immunity fragments
Aims
The aim of the experiment is to check whether we have the correct fragment sizes on the four subtilin fragments that are we have amplified yesterday.
Materials and protocol
Please refer to the gel electrophoresis protocol.
Results
Figure 1: Gel electrophoresis of the PCR products of the parts required for the subtilin immunity BioBrick.
- Lane 1: 1 kb DNA ladder
- Lane 2: Plasmid Vector (pSB1C3)
- Lane 3: Promoter and RBS (pVeg-SpoVG)
- Lane 4: spaIFEG Gene Cluster
- Lane 5: Double terminator
- Lane 6: 100 bp DNA ladder
Discussion
The correct band size were observed for for the Plasmid Vector (lane 2), Promoter & RBS (lane 3) and Double terminator (lane 5). However no band was observed for the spaIFEG(lane 4).
Conclusion
No band was observed for the spaIFEG. This could be due to the wrong melting temperature.
Go back to our main Lab book page