"
Team:UPO-Sevilla/Notebook/09 08
From 2010.igem.org
(Difference between revisions)
(New page: <div class=globalBC> {{:Team:UPO-Sevilla/header}} <!-- --> <html> <head> <script type="text/javascript" language="javascript"> <!-- current("notebook","http...) |
|||
| (2 intermediate revisions not shown) | |||
| Line 15: | Line 15: | ||
<html> | <html> | ||
| - | <h1>September, | + | <h1>September, 8th</h1> |
<h2>Production Team</h2> | <h2>Production Team</h2> | ||
| - | <p | + | <p> We performed the second overlapping PCR reaction of the SDM with the former and purified PCR products. We carried out two strategies: in one PCR reaction we used the original concentration and in another we used a 1/10 dilution of it.</p> |
<p>Products of these PCR reactions were analyzed in 0,8% gel electrophoresis and it was shown that there were not amplification.</p> | <p>Products of these PCR reactions were analyzed in 0,8% gel electrophoresis and it was shown that there were not amplification.</p> | ||
| Line 26: | Line 26: | ||
<p>Transformation of <i>E. coli</i> DH5-alfa with the products of ligations, and we spread in selective plates.</p> | <p>Transformation of <i>E. coli</i> DH5-alfa with the products of ligations, and we spread in selective plates.</p> | ||
| - | + | ||
| + | <h2>Assay Team</h2> | ||
| + | |||
| + | <p>We carried out the assay without any problem but we must wait to see the results. We have to incubate the plates overnight.</p> | ||
| + | |||
| + | <a class="return_button" href="/Team:UPO-Sevilla/Notebook/09_07" title="Go to 7th of September"><span>Go to 7th of September</span></a> | ||
| + | |||
| + | <a class="next_button" href="/Team:UPO-Sevilla/Notebook/09_09" title="Go to 9th of September"><span>Go to 9th of September</span></a> | ||
| + | |||
| + | <div class="clear"></div> | ||
| + | |||
<a class="return_button" href="/Team:UPO-Sevilla/Notebook" title="Notebook"><span>Return to Notebook</span></a> | <a class="return_button" href="/Team:UPO-Sevilla/Notebook" title="Notebook"><span>Return to Notebook</span></a> | ||
Latest revision as of 23:51, 25 October 2010
September, 8th
Production Team
We performed the second overlapping PCR reaction of the SDM with the former and purified PCR products. We carried out two strategies: in one PCR reaction we used the original concentration and in another we used a 1/10 dilution of it.
Products of these PCR reactions were analyzed in 0,8% gel electrophoresis and it was shown that there were not amplification.
Assembly Team
Transformation of E. coli DH5-alfa with the products of ligations, and we spread in selective plates.
Assay Team
We carried out the assay without any problem but we must wait to see the results. We have to incubate the plates overnight.
Go to 7th of September Go to 9th of September Return to Notebook
Sponsors
