Team:Warsaw/Stage1/ExpressIt
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<li>just one step to clone any protein coding BioBrick and you have expression construct ready</li> | <li>just one step to clone any protein coding BioBrick and you have expression construct ready</li> | ||
<li>wide spectrum of expression strengths</li> | <li>wide spectrum of expression strengths</li> | ||
- | <li>ampicillin resistance | + | <li>ampicillin resistance - this is important for constructs that use T7 promoter. Why? |
+ | T7 promoter is active ONLY in presence of T7 polymerase. Therefore you have to use a <i>E.Coli</i> strain containing this gene. The most popular choice <i>E.coli</i> BL21, which has a chloramphenicol resistance gene in the genome.</li> | ||
<li>high copy ColE1 ori (based on <a href="http://partsregistry.org/Part:pSB1A2">pSB1A2</a> vector)</li> | <li>high copy ColE1 ori (based on <a href="http://partsregistry.org/Part:pSB1A2">pSB1A2</a> vector)</li> | ||
- | <li>terminator included in vector sequence</li> | + | <li>terminator included in the vector sequence - pSB1A2 based.</li> |
</ul> | </ul> | ||
Revision as of 23:23, 25 October 2010
ExpressIt! - family of expression vectors with defined strength
Key features:
- 10 different measured RBS variants
- each variant in both constitutive (J23100 driven) and inducible (pT7 driven) version
- just one step to clone any protein coding BioBrick and you have expression construct ready
- wide spectrum of expression strengths
- ampicillin resistance - this is important for constructs that use T7 promoter. Why? T7 promoter is active ONLY in presence of T7 polymerase. Therefore you have to use a E.Coli strain containing this gene. The most popular choice E.coli BL21, which has a chloramphenicol resistance gene in the genome.
- high copy ColE1 ori (based on pSB1A2 vector)
- terminator included in the vector sequence - pSB1A2 based.
Relative strength of ExpressIt! vector family: