"
Team:Newcastle/1 September 2010
From 2010.igem.org
(Difference between revisions)
Shethharsh08 (Talk | contribs) (→Natto Rehydration) |
Shethharsh08 (Talk | contribs) (→Aims) |
||
| Line 5: | Line 5: | ||
==Aims== | ==Aims== | ||
| - | We aimed to rehydrate and plate the | + | We aimed to rehydrate and plate the sphaericus strain bought from BCCM. We used nutrient media to rehydrate the cells. The cells are going to be used to test the strength of Levans glue. |
==Materials== | ==Materials== | ||
Revision as of 22:55, 25 October 2010
| |||||||||||||
| |||||||||||||
Bacillus sphaericus Rehydration
Aims
We aimed to rehydrate and plate the sphaericus strain bought from BCCM. We used nutrient media to rehydrate the cells. The cells are going to be used to test the strength of Levans glue.
Materials
- Pennassay G- Thy medium:
- Pennassay broth- 17.5g
- Glucose- 20g
- Thymine- 0.05g
- Distilled water- 1000ml
- Pennassay broth( per l of water):
- peptone- 10g
- Yeast extract- 1.5g
- Sodium chloride- 3.5g
- Glucose- 1.0g
- Potassium phosphate dibasic- 3.68g
- Potassium phospahte monobasic- 1.32g
- Beef extract- 1.5g
ph7 store at 2-8 °C
   
|
    
|
