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Team:Newcastle/1 September 2010
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Revision as of 22:52, 25 October 2010
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Bacillus sphaericus Rehydration
Aims
We aimed to rehydrate and plate the natto strain (Bacillus subtilis ATCC 31578) bought from DSMZ, Germany. We used Penassay G- Thy Medium to rehydrate the cells. The cells are going to be used to test the strength of PGA glue.
Materials
- Pennassay G- Thy medium:
- Pennassay broth- 17.5g
- Glucose- 20g
- Thymine- 0.05g
- Distilled water- 1000ml
- Pennassay broth( per l of water):
- peptone- 10g
- Yeast extract- 1.5g
- Sodium chloride- 3.5g
- Glucose- 1.0g
- Potassium phosphate dibasic- 3.68g
- Potassium phospahte monobasic- 1.32g
- Beef extract- 1.5g
ph7 store at 2-8 °C
   
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