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| - | Modern microbial engineering methods allow the introduction of useful exogenous metabolic pathways into cells. Metabolism of certain organic compounds is sometimes limited by the production of toxic intermediates. Several bacteria have evolved protein based microcompartments capable of sequestering such reactions, thus protecting cytosolic machinery and processes from interference by these intermediates. For our project, we will identify and transform the genes encoding proteins responsible for the production and assembly of bacterial microcompartment. Additionally, we will confirm the signal sequences that target enzymes to the protein compartments by fusing this sequence to reporter genes. To demonstrate the microcompartment’s potential to serve as nanobioreactors, we will target genes encoding a short catabolic pathway into recombinant microcompartments assembled in E. coli. | + | Modern microbial engineering methods allow the introduction of useful exogenous metabolic pathways into cells. Metabolism of certain organic compounds is sometimes limited by the production of toxic intermediates. Several bacteria have evolved protein based microcompartments capable of sequestering such reactions, thus protecting cytosolic machinery and processes from interference by these intermediates. We have identified, cloned and transformed the genes encoding proteins responsible for the production and assembly of bacterial microcompartments. Additionally, we have determined the signal sequences that targets enzymes to the protein compartments and verified this by fusing the sequence to GFP and observing that this causes the GFP to localize to the compartment |
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Revision as of 22:14, 25 October 2010
Welcome!
Welcome to the Team Minnesota Wiki for iGEM 2010!
We are a team of undergraduate students along with many faculty and post-doc advisors. This is the third year that Minnesota has sent a team to iGEM.
Metabolic Engineering: In vivo Nanobioreactors
Modern microbial engineering methods allow the introduction of useful exogenous metabolic pathways into cells. Metabolism of certain organic compounds is sometimes limited by the production of toxic intermediates. Several bacteria have evolved protein based microcompartments capable of sequestering such reactions, thus protecting cytosolic machinery and processes from interference by these intermediates. We have identified, cloned and transformed the genes encoding proteins responsible for the production and assembly of bacterial microcompartments. Additionally, we have determined the signal sequences that targets enzymes to the protein compartments and verified this by fusing the sequence to GFP and observing that this causes the GFP to localize to the compartment
Highlights for the Judges
Judges! Please navigate to our [1] page for a comprehensive list of UMN's fulfilled medal requirements and links throughout the wiki.
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