Team:UNIPV-Pavia/Material Methods/Measurements/Tecan/test1ottobre

From 2010.igem.org

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<html><p align="center"><font size="5"><b>25° TEST</b></font><br><br><font size="4">[OCTOBER, 1ST]</font></p></html><hr><br>
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==PLATE==
==PLATE==
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<br>
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* <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300022</partinfo> (wiki name: I9)
* <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300022</partinfo> (wiki name: I9)
* <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300023</partinfo> (wiki name: I10)
* <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300023</partinfo> (wiki name: I10)
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* <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300012</partinfo> (wiki name: I12)
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* <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300019</partinfo> (wiki name: I12)
* <partinfo>pSB1A2</partinfo> - <partinfo>K173001</partinfo> (positive control)
* <partinfo>pSB1A2</partinfo> - <partinfo>K173001</partinfo> (positive control)
* <partinfo>pSB1A2</partinfo> - <partinfo>BBa_B0032</partinfo> (negative control)
* <partinfo>pSB1A2</partinfo> - <partinfo>BBa_B0032</partinfo> (negative control)
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* <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300021</partinfo> (wiki name: I8-4C5)
* <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300021</partinfo> (wiki name: I8-4C5)
* <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300023</partinfo> (wiki name: I10-4C5)
* <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300023</partinfo> (wiki name: I10-4C5)
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* <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300012</partinfo> (wiki name: I12-4C5)
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* <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300019</partinfo> (wiki name: I12-4C5)
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==RESULTS (?)==
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==RESULTS==
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Data were analyzed following the [[Team:UNIPV-Pavia/Parts/Characterization#Data analysis for self-inducible promoters (initiation-treshold determination)| data analysis procedure]] to evaluate the ODstart. The plots correpsoning to the parts tested are reported:
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[[Image:UNIPV_Pavia_FluoVSODHC0110.png| 620px | center ]]
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[[Image:UNIPV_Pavia_FluoVSODCo0110.png| 620px | center ]]
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[[Image:UNIPV_Pavia_FluoVSODLC0110.png| 620px | center ]]
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The ODstart are reported in the table below:
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{| border='1' align='center'
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|| Part || ODstart
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|-
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|| <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300024</partinfo> || 0.0260
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|-
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|| <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300021</partinfo> || 0.1024
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|-
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|| <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300022</partinfo> || 0.3933
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|-
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|| <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300023</partinfo> || 0.3466
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|-
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|| <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300019</partinfo> || 0.3213
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|-
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|| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300030</partinfo> || 0.0522
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|-
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|| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300028</partinfo> || 0.1655
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|-
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|| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300029</partinfo> || -
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|-
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|| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300025</partinfo> || 0.1058
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|-
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|| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300026</partinfo> || 0.5087
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|-
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|| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300027</partinfo> || 0.3559
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|-
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|| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300024</partinfo> || 0.2407
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|-
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|| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300021</partinfo> || -
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|-
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|| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300023</partinfo> || -
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|-
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|| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300019</partinfo> || -
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|}
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{{UNIPV-Pavia/Menu_measurments}}
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[[Team:UNIPV-Pavia/Material_Methods/Measurements|MEASUREMENTS]]
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[[Team:UNIPV-Pavia/Material_Methods/Measurements/Tecan|Tecan Test]]
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Latest revision as of 21:31, 25 October 2010
Home
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OCTOBER, 1ST


PLATE


UNIPV Pavia piastra1ottobre.png


EXPERIMENT DESCRIPTION

Motivation

The experiment was performed to evaluate the initiation transcription point (in term of absorbance) for self-inducible devices.

Methods

The experiment was performed in M9 according to Protocol 1 on the following measurement parts:

  • <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300024</partinfo> (wiki name: I7)
  • <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300021</partinfo> (wiki name: I8)
  • <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300022</partinfo> (wiki name: I9)
  • <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300023</partinfo> (wiki name: I10)
  • <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300019</partinfo> (wiki name: I12)
  • <partinfo>pSB1A2</partinfo> - <partinfo>K173001</partinfo> (positive control)
  • <partinfo>pSB1A2</partinfo> - <partinfo>BBa_B0032</partinfo> (negative control)
  • <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300030</partinfo> (wiki name I14) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo>
  • <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300028</partinfo> (wiki name I15) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo>
  • <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300029</partinfo> (wiki name I16) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo>
  • <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300025</partinfo> (wiki name I17) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo>
  • <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300026</partinfo> (wiki name I18) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo>
  • <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300027</partinfo> (wiki name I19) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo>
  • <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo> (negative control)
  • <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300024</partinfo> (wiki name: I7-4C5)
  • <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300021</partinfo> (wiki name: I8-4C5)
  • <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300023</partinfo> (wiki name: I10-4C5)
  • <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300019</partinfo> (wiki name: I12-4C5)

RESULTS

Data were analyzed following the data analysis procedure to evaluate the ODstart. The plots correpsoning to the parts tested are reported:

UNIPV Pavia FluoVSODHC0110.png
UNIPV Pavia FluoVSODCo0110.png
UNIPV Pavia FluoVSODLC0110.png


The ODstart are reported in the table below:

Part ODstart
<partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300024</partinfo> 0.0260
<partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300021</partinfo> 0.1024
<partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300022</partinfo> 0.3933
<partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300023</partinfo> 0.3466
<partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300019</partinfo> 0.3213
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300030</partinfo> 0.0522
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300028</partinfo> 0.1655
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300029</partinfo> -
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300025</partinfo> 0.1058
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300026</partinfo> 0.5087
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300027</partinfo> 0.3559
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300024</partinfo> 0.2407
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300021</partinfo> -
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300023</partinfo> -
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300019</partinfo> -



Previous Test

Tecan Test

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