Team:UNIPV-Pavia/Material Methods/Measurements/Tecan/test1ottobre

From 2010.igem.org

(Difference between revisions)
(New page: __NOTOC__ <table width="100%" border="0"> <tr> <td colspan="3"> {{UNIPV-Pavia/header}} </td> <!-- <td colspan="3" align="center"><html><img src="http://img337.imageshack.us...)
 
(4 intermediate revisions not shown)
Line 11: Line 11:
<td valign="top">
<td valign="top">
<table border="0" align="center" width="100%"><tr><td align="justify" valign="top" style="padding:20px">
<table border="0" align="center" width="100%"><tr><td align="justify" valign="top" style="padding:20px">
-
<html><p align="center"><font size="5"><b>25° TEST</b></font><br><br><font size="4">[OCTOBER, 1ST]</font></p></html><hr><br>
+
<html><p align="center"><font size="5"><b>OCTOBER, 1ST</b></font></p></html><hr><br>
==PLATE==
==PLATE==
<br>
<br>
Line 28: Line 28:
* <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300022</partinfo> (wiki name: I9)
* <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300022</partinfo> (wiki name: I9)
* <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300023</partinfo> (wiki name: I10)
* <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300023</partinfo> (wiki name: I10)
-
* <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300012</partinfo> (wiki name: I12)
+
* <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300019</partinfo> (wiki name: I12)
* <partinfo>pSB1A2</partinfo> - <partinfo>K173001</partinfo> (positive control)
* <partinfo>pSB1A2</partinfo> - <partinfo>K173001</partinfo> (positive control)
* <partinfo>pSB1A2</partinfo> - <partinfo>BBa_B0032</partinfo> (negative control)
* <partinfo>pSB1A2</partinfo> - <partinfo>BBa_B0032</partinfo> (negative control)
Line 41: Line 41:
* <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300021</partinfo> (wiki name: I8-4C5)
* <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300021</partinfo> (wiki name: I8-4C5)
* <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300023</partinfo> (wiki name: I10-4C5)
* <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300023</partinfo> (wiki name: I10-4C5)
-
* <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300012</partinfo> (wiki name: I12-4C5)
+
* <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300019</partinfo> (wiki name: I12-4C5)
-
==RESULTS (?)==
+
==RESULTS==
 +
Data were analyzed following the [[Team:UNIPV-Pavia/Parts/Characterization#Data analysis for self-inducible promoters (initiation-treshold determination)| data analysis procedure]] to evaluate the ODstart. The plots correpsoning to the parts tested are reported:
 +
[[Image:UNIPV_Pavia_FluoVSODHC0110.png| 620px | center ]]
 +
 +
[[Image:UNIPV_Pavia_FluoVSODCo0110.png| 620px | center ]]
 +
 +
[[Image:UNIPV_Pavia_FluoVSODLC0110.png| 620px | center ]]
 +
 +
 +
The ODstart are reported in the table below:
 +
 +
{| border='1' align='center'
 +
|| Part || ODstart
 +
|-
 +
|| <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300024</partinfo> || 0.0260
 +
|-
 +
|| <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300021</partinfo> || 0.1024
 +
|-
 +
|| <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300022</partinfo> || 0.3933
 +
|-
 +
|| <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300023</partinfo> || 0.3466
 +
|-
 +
|| <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300019</partinfo> || 0.3213
 +
|-
 +
|| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300030</partinfo> || 0.0522
 +
|-
 +
|| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300028</partinfo> || 0.1655
 +
|-
 +
|| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300029</partinfo> || -
 +
|-
 +
|| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300025</partinfo> || 0.1058
 +
|-
 +
|| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300026</partinfo> || 0.5087
 +
|-
 +
|| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300027</partinfo> || 0.3559
 +
|-
 +
|| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300024</partinfo> || 0.2407
 +
|-
 +
|| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300021</partinfo> || -
 +
|-
 +
|| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300023</partinfo> || -
 +
|-
 +
|| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300019</partinfo> || -
 +
|}
<!-- table previous next test -->
<!-- table previous next test -->
<br><br>
<br><br>
Line 59: Line 102:
<td align="left" valign="top" width="15%">
<td align="left" valign="top" width="15%">
-
<table border="0" width="100%" height="100%">
+
{{UNIPV-Pavia/Menu_measurments}}
-
<tr align="right"><td height="15">
+
-
[[Team:UNIPV-Pavia/Material_Methods/Measurements|MEASUREMENTS]]
+
-
</td></tr>
+
-
<tr align="right"><td height="15">
+
-
[[Team:UNIPV-Pavia/Material_Methods/Measurements/Tecan|Tecan Test]]
+
-
</td></tr>
+
-
</table>
+
</td>
</td>
</table>
</table>

Latest revision as of 21:31, 25 October 2010

OCTOBER, 1ST



PLATE


UNIPV Pavia piastra1ottobre.png


EXPERIMENT DESCRIPTION

Motivation

The experiment was performed to evaluate the initiation transcription point (in term of absorbance) for self-inducible devices.

Methods

The experiment was performed in M9 according to Protocol 1 on the following measurement parts:

  • <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300024</partinfo> (wiki name: I7)
  • <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300021</partinfo> (wiki name: I8)
  • <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300022</partinfo> (wiki name: I9)
  • <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300023</partinfo> (wiki name: I10)
  • <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300019</partinfo> (wiki name: I12)
  • <partinfo>pSB1A2</partinfo> - <partinfo>K173001</partinfo> (positive control)
  • <partinfo>pSB1A2</partinfo> - <partinfo>BBa_B0032</partinfo> (negative control)
  • <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300030</partinfo> (wiki name I14) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo>
  • <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300028</partinfo> (wiki name I15) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo>
  • <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300029</partinfo> (wiki name I16) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo>
  • <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300025</partinfo> (wiki name I17) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo>
  • <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300026</partinfo> (wiki name I18) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo>
  • <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300027</partinfo> (wiki name I19) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo>
  • <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo> (negative control)
  • <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300024</partinfo> (wiki name: I7-4C5)
  • <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300021</partinfo> (wiki name: I8-4C5)
  • <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300023</partinfo> (wiki name: I10-4C5)
  • <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300019</partinfo> (wiki name: I12-4C5)

RESULTS

Data were analyzed following the data analysis procedure to evaluate the ODstart. The plots correpsoning to the parts tested are reported:

UNIPV Pavia FluoVSODHC0110.png
UNIPV Pavia FluoVSODCo0110.png
UNIPV Pavia FluoVSODLC0110.png


The ODstart are reported in the table below:

Part ODstart
<partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300024</partinfo> 0.0260
<partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300021</partinfo> 0.1024
<partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300022</partinfo> 0.3933
<partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300023</partinfo> 0.3466
<partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300019</partinfo> 0.3213
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300030</partinfo> 0.0522
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300028</partinfo> 0.1655
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300029</partinfo> -
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300025</partinfo> 0.1058
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300026</partinfo> 0.5087
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300027</partinfo> 0.3559
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300024</partinfo> 0.2407
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300021</partinfo> -
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300023</partinfo> -
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300019</partinfo> -



Tecan Test