Team:UNIPV-Pavia/Material Methods/Measurements/Tecan/test20agosto

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(Methods)
 
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<html><p align="center"><font size="5"><b>14° TEST</b></font><br><br><font size="4">[AUGUST, 20TH]</font></p></html><hr><br>
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<html><p align="center"><font size="5"><b>AUGUST, 20TH</b></font></p></html><hr><br>
==PLATE==
==PLATE==
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<br>
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<br>
==EXPERIMENT DESCRIPTION==
==EXPERIMENT DESCRIPTION==
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==RESULTS (?)==
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===Motivation===
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The experiment was performed to evaluate the initiation transcription point (in term of absorbance) for self-inducible devices.
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===Methods===
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The experiment was performed in LB according to [[Team:UNIPV-Pavia/Parts/Characterization#Microplate reader experiments for self-inducible promoters - Protocol #1|Protocol 1]] on the following measurement parts:
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* <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300024</partinfo> (wiki name: I7)
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* <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300021</partinfo> (wiki name: I8)
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* <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300022</partinfo> (wiki name: I9)
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* <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300023</partinfo> (wiki name: I10)
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* <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300019</partinfo> (wiki name: I12)
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* <partinfo>pSB1A2</partinfo> - <partinfo>K173001</partinfo> (positive control)
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* <partinfo>pSB1A2</partinfo> - <partinfo>BBa_B0032</partinfo> (negative control)
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* <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300030</partinfo> (wiki name I14-4C5) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo>
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* <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300028</partinfo> (wiki name I15-4C5) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo>
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* <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300029</partinfo> (wiki name I16-4C5) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo>
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* <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300025</partinfo> (wiki name I17-4C5) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo>
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* <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300026</partinfo> (wiki name I18-4C5) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo>
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* <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300027</partinfo> (wiki name I19-4C5) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo>
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* <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo> (negative control)
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* <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300024</partinfo> (wiki name: I7-4C5)
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* <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300021</partinfo> (wiki name: I8-4C5)
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* <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300023</partinfo> (wiki name: I10-4C5)
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* <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300019</partinfo> (wiki name: I12-4C5)
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==RESULTS==
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Data were analyzed following the [[Team:UNIPV-Pavia/Parts/Characterization#Data analysis for self-inducible promoters (initiation-treshold determination)| data analysis procedure]] to evaluate the ODstart. The plots correpsoning to the parts tested are reported:
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[[Image:UNIPV_Pavia_FluoVSODHC2008.png| 620px | center ]]
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[[Image:UNIPV_Pavia_FluoVSODCo2008.png| 620px | center ]]
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[[Image:UNIPV_Pavia_FluoVSODLC2008.png| 620px | center ]]
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The ODstart are reported in the table below:
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{| border='1' align='center'
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|| Part || ODstart
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|-
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|| <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300024</partinfo> || 0.1292
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|-
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|| <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300021</partinfo> || -
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|-
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|| <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300022</partinfo> || -
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|-
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|| <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300023</partinfo> || -
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|-
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|| <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300019</partinfo> || 0.5210
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|-
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|| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300030</partinfo> || 0.1785
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|-
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|| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300028</partinfo> || 0.2824
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|-
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|| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300029</partinfo> || -
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|-
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|| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300025</partinfo> || 0.1957
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|-
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|| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300026</partinfo> || -
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|-
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|| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300027</partinfo> || -
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|-
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|| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300024</partinfo> || -
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|-
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|| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300021</partinfo> || -
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|-
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|| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300023</partinfo> || -
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|-
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|| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300019</partinfo> || -
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|}
<!-- table previous next test -->
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<table border="0" width="100%" class="menu">
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<td align="left" width="50%">[[Team:UNIPV-Pavia/Material Methods/Measurements/Tecan/test13|Previous Test]]</td>
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<td align="left" width="50%">[[Team:UNIPV-Pavia/Material Methods/Measurements/Tecan/test18agosto|Previous Test]]</td>
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<td align="right" width="50%">[[Team:UNIPV-Pavia/Material Methods/Measurements/Tecan/test15| Next Test]]</td>
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<td align="right" width="50%">[[Team:UNIPV-Pavia/Material Methods/Measurements/Tecan/test27agosto| Next Test]]</td>
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{{UNIPV-Pavia/Menu_measurments}}
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[[Team:UNIPV-Pavia/Material_Methods/Measurements|MEASUREMENTS]]
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[[Team:UNIPV-Pavia/Material_Methods/Measurements/Tecan|Tecan Test]]
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Latest revision as of 21:10, 25 October 2010

AUGUST, 20TH



PLATE


UNIPV Pavia piastra20agosto.png


EXPERIMENT DESCRIPTION

Motivation

The experiment was performed to evaluate the initiation transcription point (in term of absorbance) for self-inducible devices.

Methods

The experiment was performed in LB according to Protocol 1 on the following measurement parts:

  • <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300024</partinfo> (wiki name: I7)
  • <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300021</partinfo> (wiki name: I8)
  • <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300022</partinfo> (wiki name: I9)
  • <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300023</partinfo> (wiki name: I10)
  • <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300019</partinfo> (wiki name: I12)
  • <partinfo>pSB1A2</partinfo> - <partinfo>K173001</partinfo> (positive control)
  • <partinfo>pSB1A2</partinfo> - <partinfo>BBa_B0032</partinfo> (negative control)
  • <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300030</partinfo> (wiki name I14-4C5) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo>
  • <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300028</partinfo> (wiki name I15-4C5) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo>
  • <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300029</partinfo> (wiki name I16-4C5) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo>
  • <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300025</partinfo> (wiki name I17-4C5) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo>
  • <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300026</partinfo> (wiki name I18-4C5) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo>
  • <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300027</partinfo> (wiki name I19-4C5) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo>
  • <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo> (negative control)
  • <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300024</partinfo> (wiki name: I7-4C5)
  • <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300021</partinfo> (wiki name: I8-4C5)
  • <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300023</partinfo> (wiki name: I10-4C5)
  • <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300019</partinfo> (wiki name: I12-4C5)

RESULTS

Data were analyzed following the data analysis procedure to evaluate the ODstart. The plots correpsoning to the parts tested are reported:

UNIPV Pavia FluoVSODHC2008.png
UNIPV Pavia FluoVSODCo2008.png
UNIPV Pavia FluoVSODLC2008.png

The ODstart are reported in the table below:

Part ODstart
<partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300024</partinfo> 0.1292
<partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300021</partinfo> -
<partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300022</partinfo> -
<partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300023</partinfo> -
<partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300019</partinfo> 0.5210
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300030</partinfo> 0.1785
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300028</partinfo> 0.2824
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300029</partinfo> -
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300025</partinfo> 0.1957
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300026</partinfo> -
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300027</partinfo> -
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300024</partinfo> -
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300021</partinfo> -
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300023</partinfo> -
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300019</partinfo> -



Tecan Test