Team:UNIPV-Pavia/Material Methods/Measurements/Tecan/test17agosto

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<html><p align="center"><font size="5"><b>12° TEST</b></font><br><br><font size="4">[AUGUST, 17TH]</font></p></html><hr><br>
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<html><p align="center"><font size="5"><b>AUGUST, 17TH</b></font></p></html><hr><br>
==PLATE==
==PLATE==
<br>
<br>
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* <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300022</partinfo> (wiki name: I9)
* <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300022</partinfo> (wiki name: I9)
* <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300023</partinfo> (wiki name: I10)
* <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300023</partinfo> (wiki name: I10)
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* <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300012</partinfo> (wiki name: I12)
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* <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300019</partinfo> (wiki name: I12)
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* <partinfo>pSB1A2</partinfo> - <partinfo>K173001</partinfo (positive control)
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* <partinfo>pSB1A2</partinfo> - <partinfo>K173001</partinfo> (positive control)
* <partinfo>pSB1A2</partinfo> - <partinfo>BBa_B0032</partinfo> (negative control)
* <partinfo>pSB1A2</partinfo> - <partinfo>BBa_B0032</partinfo> (negative control)
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* <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300030</partinfo> (wiki name I14) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo>
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* <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300030</partinfo> (wiki name I14-4C5) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo>
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* <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300029</partinfo> (wiki name I16) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo>
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* <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300029</partinfo> (wiki name I16-4C5) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo>
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* <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300025</partinfo> (wiki name I17) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo>
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* <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300025</partinfo> (wiki name I17-4C5) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo>
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* <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300026</partinfo> (wiki name I18) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo>
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* <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300026</partinfo> (wiki name I18-4C5) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo>
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* <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300027</partinfo> (wiki name I19) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo>
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* <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300027</partinfo> (wiki name I19-4C5) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo>
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* <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo>
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* <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo> (negative control)
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==RESULTS (?)==
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==RESULTS==
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Data were analyzed following the [[Team:UNIPV-Pavia/Parts/Characterization#Data analysis for self-inducible promoters (initiation-treshold determination)| data analysis procedure]] to evaluate the ODstart. The plots correpsoning to the parts tested are reported:
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[[Image:UNIPV_Pavia_FluoVSODHC1708.png| 620px | center ]]
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[[Image:UNIPV_Pavia_FluoVSODCo1708.png| 620px | center ]]
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The ODstart are reported in the table below:
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{| border='1' align='center'
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|| Part || ODstart
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|-
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|| <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300024</partinfo> || 0.1456
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|-
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|| <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300021</partinfo> || 0
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|-
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|| <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300022</partinfo> || 0.3257
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|-
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|| <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300023</partinfo> || -
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|-
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|| <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300019</partinfo> || 0.5068
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|-
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|| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300030</partinfo> || 0.1609
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|-
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|| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300029</partinfo> || 0.3098
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|-
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|| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300025</partinfo> || 0.1463
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|-
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|| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300026</partinfo> || -
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|-
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|| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300027</partinfo> || -
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|}
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<td align="left" width="50%">[[Team:UNIPV-Pavia/Material Methods/Measurements/Tecan/test10agosto|Previous Test]]</td>
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<td align="left" width="50%"></td>
<td align="right" width="50%">[[Team:UNIPV-Pavia/Material Methods/Measurements/Tecan/test18agosto| Next Test]]</td>
<td align="right" width="50%">[[Team:UNIPV-Pavia/Material Methods/Measurements/Tecan/test18agosto| Next Test]]</td>
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{{UNIPV-Pavia/Menu_measurments}}
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[[Team:UNIPV-Pavia/Material_Methods/Measurements|MEASUREMENTS]]
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[[Team:UNIPV-Pavia/Material_Methods/Measurements/Tecan|Tecan Test]]
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Latest revision as of 21:08, 25 October 2010
Home
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AUGUST, 17TH


PLATE


UNIPV Pavia piastra17agosto.png


EXPERIMENT DESCRIPTION

Motivation

The experiment was performed to evaluate the initiation transcription point (in term of absorbance) for self-inducible devices.

Methods

The experiment was performed in LB according to Protocol 1 on the following measurement parts:

  • <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300024</partinfo> (wiki name: I7)
  • <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300021</partinfo> (wiki name: I8)
  • <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300022</partinfo> (wiki name: I9)
  • <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300023</partinfo> (wiki name: I10)
  • <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300019</partinfo> (wiki name: I12)
  • <partinfo>pSB1A2</partinfo> - <partinfo>K173001</partinfo> (positive control)
  • <partinfo>pSB1A2</partinfo> - <partinfo>BBa_B0032</partinfo> (negative control)
  • <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300030</partinfo> (wiki name I14-4C5) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo>
  • <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300029</partinfo> (wiki name I16-4C5) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo>
  • <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300025</partinfo> (wiki name I17-4C5) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo>
  • <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300026</partinfo> (wiki name I18-4C5) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo>
  • <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300027</partinfo> (wiki name I19-4C5) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo>
  • <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo> (negative control)

RESULTS

Data were analyzed following the data analysis procedure to evaluate the ODstart. The plots correpsoning to the parts tested are reported:

UNIPV Pavia FluoVSODHC1708.png
UNIPV Pavia FluoVSODCo1708.png

The ODstart are reported in the table below:

Part ODstart
<partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300024</partinfo> 0.1456
<partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300021</partinfo> 0
<partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300022</partinfo> 0.3257
<partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300023</partinfo> -
<partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300019</partinfo> 0.5068
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300030</partinfo> 0.1609
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300029</partinfo> 0.3098
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300025</partinfo> 0.1463
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300026</partinfo> -
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300027</partinfo> -



Next Test

Tecan Test

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