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Team:UNIPV-Pavia/Material Methods/Measurements/Tecan/test17agosto
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| - | <html><p align="center"><font size="5"><b> | + | <html><p align="center"><font size="5"><b>AUGUST, 17TH</b></font></p></html><hr><br> |
==PLATE== | ==PLATE== | ||
<br> | <br> | ||
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===Methods=== | ===Methods=== | ||
| - | + | The experiment was performed in LB according to [[Team:UNIPV-Pavia/Parts/Characterization#Microplate reader experiments for self-inducible promoters - Protocol #1|Protocol 1]] on the following measurement parts: | |
| - | [[Team:UNIPV-Pavia/Parts/Characterization#Microplate reader experiments for self-inducible promoters - Protocol #1|Protocol 1]] | + | |
| - | ==RESULTS ( | + | * <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300024</partinfo> (wiki name: I7) |
| + | * <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300021</partinfo> (wiki name: I8) | ||
| + | * <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300022</partinfo> (wiki name: I9) | ||
| + | * <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300023</partinfo> (wiki name: I10) | ||
| + | * <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300019</partinfo> (wiki name: I12) | ||
| + | * <partinfo>pSB1A2</partinfo> - <partinfo>K173001</partinfo> (positive control) | ||
| + | * <partinfo>pSB1A2</partinfo> - <partinfo>BBa_B0032</partinfo> (negative control) | ||
| + | * <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300030</partinfo> (wiki name I14-4C5) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo> | ||
| + | * <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300029</partinfo> (wiki name I16-4C5) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo> | ||
| + | * <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300025</partinfo> (wiki name I17-4C5) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo> | ||
| + | * <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300026</partinfo> (wiki name I18-4C5) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo> | ||
| + | * <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300027</partinfo> (wiki name I19-4C5) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo> | ||
| + | * <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo> (negative control) | ||
| + | |||
| + | ==RESULTS== | ||
| + | Data were analyzed following the [[Team:UNIPV-Pavia/Parts/Characterization#Data analysis for self-inducible promoters (initiation-treshold determination)| data analysis procedure]] to evaluate the ODstart. The plots correpsoning to the parts tested are reported: | ||
| + | |||
| + | [[Image:UNIPV_Pavia_FluoVSODHC1708.png| 620px | center ]] | ||
| + | |||
| + | [[Image:UNIPV_Pavia_FluoVSODCo1708.png| 620px | center ]] | ||
| + | |||
| + | The ODstart are reported in the table below: | ||
| + | |||
| + | {| border='1' align='center' | ||
| + | || Part || ODstart | ||
| + | |- | ||
| + | || <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300024</partinfo> || 0.1456 | ||
| + | |- | ||
| + | || <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300021</partinfo> || 0 | ||
| + | |- | ||
| + | || <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300022</partinfo> || 0.3257 | ||
| + | |- | ||
| + | || <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300023</partinfo> || - | ||
| + | |- | ||
| + | || <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300019</partinfo> || 0.5068 | ||
| + | |- | ||
| + | || <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300030</partinfo> || 0.1609 | ||
| + | |- | ||
| + | || <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300029</partinfo> || 0.3098 | ||
| + | |- | ||
| + | || <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300025</partinfo> || 0.1463 | ||
| + | |- | ||
| + | || <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300026</partinfo> || - | ||
| + | |- | ||
| + | || <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300027</partinfo> || - | ||
| + | |} | ||
<!-- table previous next test --> | <!-- table previous next test --> | ||
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| - | <td align="left" width="50%"> | + | <td align="left" width="50%"></td> |
<td align="right" width="50%">[[Team:UNIPV-Pavia/Material Methods/Measurements/Tecan/test18agosto| Next Test]]</td> | <td align="right" width="50%">[[Team:UNIPV-Pavia/Material Methods/Measurements/Tecan/test18agosto| Next Test]]</td> | ||
</tr> | </tr> | ||
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| - | + | {{UNIPV-Pavia/Menu_measurments}} | |
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</table> | </table> | ||
Latest revision as of 21:08, 25 October 2010
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