Team:Newcastle/9 July 2010

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'''9 July 2010'''
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=Research=
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=LacI BioBrick Construction=
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Today we initiated with our research in urease which would lead to calcium carbonate production inside the crack.
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==Aims==
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#Wray LV, Ferson E and Fisher SH. (1997). "Expression of the Bacillus subtilis ureABC operon is controlled by multiple regulatory factors including CodY, GlnR, TnrA, and Spo0H". ''Journal of bacteriology''. 179(17). 5494-501.
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* To use PCR to extract ''lacI'' (promoter, ribosome-binding site (RBS) & coding sequence (CDS)) from plasmid pMutin4 and ligate into vector pSB1AT3 in front of red fluorescent protein (RFP).
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#Kim JK, Mulrooney SB and Hausinger RP. (2005). "Biosynthesis of Active Bacillus subtilis Urease in the Absence of Known Urease Accessory Proteins". ''Journal of Bacteriology''. 187(20). 7150-7154.  
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==Materials==
 
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* Competent ''E. coli'' (DH5alpha strain)
 
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* Ligation of ''lacI'' into pSB1AT3
 
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==Protocol==
 
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* [[TeamNewcastleTransformation of E. coli|Transformation of ''E. coli'']] DH5alpha pSB1AT3 containing ''lacI'' insert.
 
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==Inference==
 
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*''Ecoli'' DH5alpha is used as a bioreactor to replicate our plasmids to create a large stock ready for extraction from cells.
 
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Latest revision as of 19:16, 25 October 2010

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Research

Today we initiated with our research in urease which would lead to calcium carbonate production inside the crack.

  1. Wray LV, Ferson E and Fisher SH. (1997). "Expression of the Bacillus subtilis ureABC operon is controlled by multiple regulatory factors including CodY, GlnR, TnrA, and Spo0H". Journal of bacteriology. 179(17). 5494-501.
  2. Kim JK, Mulrooney SB and Hausinger RP. (2005). "Biosynthesis of Active Bacillus subtilis Urease in the Absence of Known Urease Accessory Proteins". Journal of Bacteriology. 187(20). 7150-7154.


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