Team:Heidelberg/Modeling/miBSdesigner

From 2010.igem.org

(Difference between revisions)
Line 8: Line 8:
<style type="text/css">
<style type="text/css">
div.wrapper {width: 260px; height: 650px; border-width:3px; margin:auto; border-style:solid; border-color:#11526f;  text-align:center;}
div.wrapper {width: 260px; height: 650px; border-width:3px; margin:auto; border-style:solid; border-color:#11526f;  text-align:center;}
-
div.wrapper2 {padding:25px; border-width:3px; margin:auto; border-style:solid; border-color:#11526f; height: 650px; text-align:left; }
+
div.wrapper2 {padding:20px; border-width:3px; margin:auto; border-style:solid; border-color:#11526f; height: 660px; text-align:left; }
div.title {text-indent:30px;}
div.title {text-indent:30px;}
div.option {text-indent:50px; white-space:pre-wrap;}
div.option {text-indent:50px; white-space:pre-wrap;}
Line 24: Line 24:
<center>
<center>
<span style="font-family: Arial; color: #11526f; font-size: 18.0px; font-weight: bold;">
<span style="font-family: Arial; color: #11526f; font-size: 18.0px; font-weight: bold;">
-
Construction of microRNA binding sites </span>
+
Construction of microRNA binding sites </span><br>
<span style="font-size: 17.0px;">Sequence name </span>
<span style="font-size: 17.0px;">Sequence name </span>
<br>
<br>
Line 122: Line 122:
{{:Team:Heidelberg/Pagemiddle}}
{{:Team:Heidelberg/Pagemiddle}}
<html>
<html>
-
<div class="wrapper"><br>
+
<div class="wrapper">
<img style="border-width: 0px;" src="https://static.igem.org/mediawiki/2010/c/c0/MiBSdesigner.png" width="260" height="147" />
<img style="border-width: 0px;" src="https://static.igem.org/mediawiki/2010/c/c0/MiBSdesigner.png" width="260" height="147" />
<br><br>
<br><br>

Revision as of 18:57, 25 October 2010

BS designer for miRNA
Construction of microRNA binding sites
Sequence name

microRNA Sequence ( 5' -> 3')

Spacer (inert sequence)


Perfect Binding Site
Imperfect Binding Site with 4 nt bulge (9-12)
Personalized Binding Site

Seed sequence (1-8)
Customized MISMATCH position

Supplementary region
Customized MATCH positions (input numbers from 9 to 22)


Modify AU content
A in position -1
A in position 0
A in position 1
A in position 8
A in position 9
A in position 10


Sticky ends for integration in plasmid:
Customized: 5' 3'







Name of primer 1


Sequence of primer 1


Name of primer 2


Sequence of primer 2