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Team:Newcastle/Meetings/12 July 2010
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| - | == | + | ==Informal meeting - 12th July 2010== |
| - | + | *Chair: Da, Minutes: Younus | |
| - | + | *Walton Library [3.30 - 5.00pm] | |
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| + | ===Cloning Strategies=== | ||
| - | ==== | + | =====5 parts to a Cloning Strategy:===== |
| - | + | *The cloning strategies for each biobrick should contain the following sections in this order: | |
| + | *#Purpose and Justification | ||
| + | *#Modelling | ||
| + | *#Construction | ||
| + | *#Cloning and Integration | ||
| + | *#Testing and Characterising | ||
| - | *'''SR1''' - finished! | + | =====Required Cloning Strategies===== |
| - | *'''YneA''' - biobrick nearly coming back - our most important biobrick! | + | *There are 8 cloning strategies which are required: |
| - | *'''LacI''' - not sure how to test this biobrick | + | **'''SR1''' - finished! |
| - | *'''2 for Swarming''' (sfp and swrA) - cloning strategies nearly finished | + | **'''YneA''' - biobrick nearly coming back - our most important biobrick! |
| - | *'''2 for Subtilin''' (immunity and production) - cloning strategies nearly finished | + | **'''LacI''' - not sure how to test this biobrick |
| - | *'''RocF''' (need to use Gibson method) - not started | + | **'''2 for Swarming''' (sfp and swrA) - cloning strategies nearly finished |
| + | **'''2 for Subtilin''' (immunity and production) - cloning strategies nearly finished | ||
| + | **'''RocF''' (need to use Gibson method) - not started | ||
So only the cloning strategy for SR1 is finished. 7 more to go! | So only the cloning strategy for SR1 is finished. 7 more to go! | ||
| - | + | =====Allocation of team members to cloning strategies:===== | |
| - | ====Allocation of team members to cloning strategies:==== | + | |
*'''YneA''' - Phil, Rach, Deena, Younus | *'''YneA''' - Phil, Rach, Deena, Younus | ||
| + | *'''LacI''' - Phil, Younus, Rachel | ||
*'''Swarming (2)''' - Harsh, Alan, Steve, Da | *'''Swarming (2)''' - Harsh, Alan, Steve, Da | ||
| - | |||
*'''Subtilin (2)''' - Harsh, Alan, Steve, Deena | *'''Subtilin (2)''' - Harsh, Alan, Steve, Deena | ||
*'''RocF''' - Harsh, Alan, Steve, Da | *'''RocF''' - Harsh, Alan, Steve, Da | ||
| - | + | ====Wiki - Labbook==== | |
| - | + | ||
| - | + | ||
| - | ====Labbook==== | + | |
Everyone needs to carry out the following protocol for each day in the lab | Everyone needs to carry out the following protocol for each day in the lab | ||
#Aim | #Aim | ||
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*Wiki checkers: Deena and Rachel (after altering/updating a page on the wiki, team members should send the links to the pages to the wiki checkers) | *Wiki checkers: Deena and Rachel (after altering/updating a page on the wiki, team members should send the links to the pages to the wiki checkers) | ||
| - | + | ===UK iGEM Gathering at Newcastle - 20th/21st July=== | |
| - | + | ====Presentation:==== | |
| - | == | + | =====Introduction===== |
| - | + | *Rach, Deena, Jannetta [2 mins] | |
| - | ===Presentation:=== | + | **Cover Page |
| - | ====Introduction==== | + | **Picture of us standing up |
| - | + | **Introduction of our iGEM project - using BS to repair concrete | |
| - | *Cover Page | + | =====General Outlook===== |
| - | *Picture of us standing up | + | *Harsh, Da, Phil [5 mins] |
| - | *Introduction of our iGEM project - using BS to repair concrete | + | **Starting with bioreactor |
| - | + | **Transformation into spores | |
| - | ====General Outlook==== | + | **Transportation |
| - | + | **Spraying on building | |
| - | *Starting with bioreactor | + | **Germination |
| - | *Transformation into spores | + | **sensing crack + non-environmental kill switch |
| - | *Transportation | + | **swarming |
| - | *Spraying on building | + | **end of crack signalling = QS |
| - | *Germination | + | **Filamentous cells |
| - | *sensing crack + non-environmental kill switch | + | **CaCO3 |
| - | *swarming | + | =====BioBrick Discussion===== |
| - | *end of crack signalling = QS | + | *Alan, Younus, Steve [8 mins altogether; 2 mins each] |
| - | *Filamentous cells | + | **Swarming |
| - | *CaCO3 | + | **Subtillin |
| - | + | **Filamentous cells - YneA | |
| - | + | **CaCO3 - Urease | |
| - | + | **LacI | |
| - | ====BioBrick Discussion==== | + | =====Whats next...===== |
| - | [8 mins altogether; 2 mins each] | + | *[5 mins remaining] |
| - | *Swarming | + | **'Save the babies!!!' |
| - | *Subtillin | + | |
| - | *Filamentous cells - YneA | + | |
| - | *CaCO3 - Urease | + | |
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | ====Whats next...==== | + | |
| - | 'Save the babies!!!' | + | |
| - | + | ||
| - | + | ||
| - | ==Minideadlines | + | ===Minideadlines=== |
| - | #Cloning Strategies | + | #Presentation slides to be e-mailed by tomorrow (Wed) 5 pm |
| + | #Cloning Strategies - to be completed by Friday, 16th July 2010. | ||
#*YneA | #*YneA | ||
#*One sequence for sfp primers and coding sequence ready (Goksel) | #*One sequence for sfp primers and coding sequence ready (Goksel) | ||
#*Subtilin just immunity | #*Subtilin just immunity | ||
| - | ==Action Points== | + | ===Action Points=== |
#Finish wiki updates by Sunday 18th (everyone) | #Finish wiki updates by Sunday 18th (everyone) | ||
#Team page on Wiki: | #Team page on Wiki: | ||
| - | #*Add Colin Harwood, Colin | + | #*Add Colin Harwood, Colin Davies, Wendy Smith and Goksel Misrili to list of advisors |
#*Deena, Phil and Younus to write about yourself + pictures | #*Deena, Phil and Younus to write about yourself + pictures | ||
Latest revision as of 18:34, 25 October 2010
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Contents |
Informal meeting - 12th July 2010
- Chair: Da, Minutes: Younus
- Walton Library [3.30 - 5.00pm]
Cloning Strategies
5 parts to a Cloning Strategy:
- The cloning strategies for each biobrick should contain the following sections in this order:
- Purpose and Justification
- Modelling
- Construction
- Cloning and Integration
- Testing and Characterising
Required Cloning Strategies
- There are 8 cloning strategies which are required:
- SR1 - finished!
- YneA - biobrick nearly coming back - our most important biobrick!
- LacI - not sure how to test this biobrick
- 2 for Swarming (sfp and swrA) - cloning strategies nearly finished
- 2 for Subtilin (immunity and production) - cloning strategies nearly finished
- RocF (need to use Gibson method) - not started
So only the cloning strategy for SR1 is finished. 7 more to go!
Allocation of team members to cloning strategies:
- YneA - Phil, Rach, Deena, Younus
- LacI - Phil, Younus, Rachel
- Swarming (2) - Harsh, Alan, Steve, Da
- Subtilin (2) - Harsh, Alan, Steve, Deena
- RocF - Harsh, Alan, Steve, Da
Wiki - Labbook
Everyone needs to carry out the following protocol for each day in the lab
- Aim
- Materials and Methods
- Procedure/Protocol
- Results
- Conclusion
Relevant photographs should be added and labelled
General:
- Wiki checkers: Deena and Rachel (after altering/updating a page on the wiki, team members should send the links to the pages to the wiki checkers)
UK iGEM Gathering at Newcastle - 20th/21st July
Presentation:
Introduction
- Rach, Deena, Jannetta [2 mins]
- Cover Page
- Picture of us standing up
- Introduction of our iGEM project - using BS to repair concrete
General Outlook
- Harsh, Da, Phil [5 mins]
- Starting with bioreactor
- Transformation into spores
- Transportation
- Spraying on building
- Germination
- sensing crack + non-environmental kill switch
- swarming
- end of crack signalling = QS
- Filamentous cells
- CaCO3
BioBrick Discussion
- Alan, Younus, Steve [8 mins altogether; 2 mins each]
- Swarming
- Subtillin
- Filamentous cells - YneA
- CaCO3 - Urease
- LacI
Whats next...
- [5 mins remaining]
- 'Save the babies!!!'
Minideadlines
- Presentation slides to be e-mailed by tomorrow (Wed) 5 pm
- Cloning Strategies - to be completed by Friday, 16th July 2010.
- YneA
- One sequence for sfp primers and coding sequence ready (Goksel)
- Subtilin just immunity
Action Points
- Finish wiki updates by Sunday 18th (everyone)
- Team page on Wiki:
- Add Colin Harwood, Colin Davies, Wendy Smith and Goksel Misrili to list of advisors
- Deena, Phil and Younus to write about yourself + pictures
