Team:Newcastle/Meetings/12 July 2010

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Latest revision as of 18:34, 25 October 2010

Informal meeting - 12th July 2010

Chair: Da, Minutes: Younus
Walton Library [3.30 - 5.00pm]

Cloning Strategies

5 parts to a Cloning Strategy:

The cloning strategies for each biobrick should contain the following sections in this order:
1. Purpose and Justification
2. Modelling
3. Construction
4. Cloning and Integration
5. Testing and Characterising

Required Cloning Strategies

There are 8 cloning strategies which are required:
- SR1 - finished!
- YneA - biobrick nearly coming back - our most important biobrick!
- LacI - not sure how to test this biobrick
- 2 for Swarming (sfp and swrA) - cloning strategies nearly finished
- 2 for Subtilin (immunity and production) - cloning strategies nearly finished
- RocF (need to use Gibson method) - not started

So only the cloning strategy for SR1 is finished. 7 more to go!

Allocation of team members to cloning strategies:

YneA - Phil, Rach, Deena, Younus
LacI - Phil, Younus, Rachel
Swarming (2) - Harsh, Alan, Steve, Da
Subtilin (2) - Harsh, Alan, Steve, Deena
RocF - Harsh, Alan, Steve, Da

Wiki - Labbook

Everyone needs to carry out the following protocol for each day in the lab

Aim
Materials and Methods
Procedure/Protocol
Results
Conclusion

Relevant photographs should be added and labelled

General:

Wiki checkers: Deena and Rachel (after altering/updating a page on the wiki, team members should send the links to the pages to the wiki checkers)

UK iGEM Gathering at Newcastle - 20th/21st July

Presentation:

Introduction

Rach, Deena, Jannetta [2 mins]
- Cover Page
- Picture of us standing up
- Introduction of our iGEM project - using BS to repair concrete

General Outlook

Harsh, Da, Phil [5 mins]
- Starting with bioreactor
- Transformation into spores
- Transportation
- Spraying on building
- Germination
- sensing crack + non-environmental kill switch
- swarming
- end of crack signalling = QS
- Filamentous cells
- CaCO3

BioBrick Discussion

Alan, Younus, Steve [8 mins altogether; 2 mins each]
- Swarming
- Subtillin
- Filamentous cells - YneA
- CaCO3 - Urease
- LacI

Whats next...

[5 mins remaining]
- 'Save the babies!!!'

Minideadlines

Presentation slides to be e-mailed by tomorrow (Wed) 5 pm
Cloning Strategies - to be completed by Friday, 16th July 2010.
- YneA
- One sequence for sfp primers and coding sequence ready (Goksel)
- Subtilin just immunity

Action Points

Finish wiki updates by Sunday 18th (everyone)
Team page on Wiki:
- Add Colin Harwood, Colin Davies, Wendy Smith and Goksel Misrili to list of advisors
- Deena, Phil and Younus to write about yourself + pictures

@@ Line 1: / Line 1: @@
-Chair: Da, Minutes: Younus, Comp
+{{Team:Newcastle/mainbanner}}
-#. Cloning Strategies
+==Informal meeting - 12th July 2010==
-*Finished SR1
-*RocF not started - need to use Gibson method;
-*YneA
-*2 for Swarming!
-LacI
-for Subtilin
-Only finished one, SR1. 7 more to go!
+*Chair: Da, Minutes: Younus
+*Walton Library [3.30 - 5.00pm]
-Next: swarming and subtilin nearly finished, YneA nearly coming back and LacI brick not sure how to test.
+===Cloning Strategies===
-Why is testing LacI hard?
-YneA - our most important BB - Phi, Rac, Dee
-RocF
-parts to a Cloning Strategy
+=====5 parts to a Cloning Strategy:=====
-#Purpose and justification
+*The cloning strategies for each biobrick should contain the following sections in this order:
-#Modelling
+*#Purpose and Justification
-#Construction
+*#Modelling
-#Cloning and Integration
+*#Construction
-#Testing and Characterising
+*#Cloning and Integration
+*#Testing and Characterising
+=====Required Cloning Strategies=====
+*There are 8 cloning strategies which are required:
+**'''SR1''' - finished!
+**'''YneA''' - biobrick nearly coming back - our most important biobrick!
+**'''LacI''' - not sure how to test this biobrick
+**'''2 for Swarming''' (sfp and swrA) - cloning strategies nearly finished
+**'''2 for Subtilin''' (immunity and production) - cloning strategies nearly finished
+**'''RocF''' (need to use Gibson method) - not started
-#.
+So only the cloning strategy for SR1 is finished. 7 more to go!
-#.
+=====Allocation of team members to cloning strategies:=====
+*'''YneA''' - Phil, Rach, Deena, Younus
+*'''LacI''' - Phil, Younus, Rachel
+*'''Swarming (2)''' - Harsh, Alan, Steve, Da
+*'''Subtilin (2)''' - Harsh, Alan, Steve, Deena
+*'''RocF''' - Harsh, Alan, Steve, Da
+====Wiki - Labbook====
+Everyone needs to carry out the following protocol for each day in the lab
+#Aim
+#Materials and Methods
+#Procedure/Protocol
+#Results
+#Conclusion
+Relevant photographs should be added and labelled
+====General:====
+*Wiki checkers: Deena and Rachel (after altering/updating a page on the wiki, team members should send the links to the pages to the wiki checkers)
+===UK iGEM Gathering at Newcastle - 20th/21st July===
+====Presentation:====
+=====Introduction=====
+*Rach, Deena, Jannetta [2 mins]
+**Cover Page
+**Picture of us standing up
+**Introduction of our iGEM project - using BS to repair concrete
+=====General Outlook=====
+*Harsh, Da, Phil [5 mins]
+**Starting with bioreactor
+**Transformation into spores
+**Transportation
+**Spraying on building
+**Germination
+**sensing crack + non-environmental kill switch
+**swarming
+**end of crack signalling = QS
+**Filamentous cells
+**CaCO3
+=====BioBrick Discussion=====
+*Alan, Younus, Steve [8 mins altogether; 2 mins each]
+**Swarming
+**Subtillin
+**Filamentous cells - YneA
+**CaCO3 - Urease
+**LacI
+=====Whats next...=====
+*[5 mins remaining]
+**'Save the babies!!!'
+===Minideadlines===
+#Presentation slides to be e-mailed by tomorrow (Wed) 5 pm
+#Cloning Strategies - to be completed by Friday, 16th July 2010.
+#*YneA
+#*One sequence for sfp primers and coding sequence ready (Goksel)
+#*Subtilin just immunity
+===Action Points===
+#Finish wiki updates by Sunday 18th (everyone)
+#Team page on Wiki:
+#*Add Colin Harwood, Colin Davies, Wendy Smith and Goksel Misrili to list of advisors
+#*Deena, Phil and Younus to write about yourself + pictures