Team:Newcastle/Medals
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# <strike> <span style="color:#8B4513"> '''BRONZE TWO:''' </span> </strike> Successfully complete and submit a Project Summary form. | # <strike> <span style="color:#8B4513"> '''BRONZE TWO:''' </span> </strike> Successfully complete and submit a Project Summary form. | ||
#*'''Due September 20''': see [https://2010.igem.org/Jamboree/Project_abstract]. | #*'''Due September 20''': see [https://2010.igem.org/Jamboree/Project_abstract]. | ||
- | #*'''Project Abstract''':BacillaFilla, an engineered ''Bacillus subtilis'', aims to repair [[Team:Newcastle/problem|microcracks in concrete]], which can cause catastrophic structural failure. BacillaFilla would be applied to structures by spraying onto their surface. | + | #*'''Project Abstract''': |
+ | BacillaFilla, an engineered ''Bacillus subtilis'', aims to repair [[Team:Newcastle/problem|microcracks in concrete]], which can cause catastrophic structural failure. BacillaFilla would be applied to structures by spraying onto their surface. | ||
BacillaFilla swims deep into the microcracks. Repair is effected by production of CaCO<sub>3</sub>, [[Team:Newcastle/Filamentous_Cells| filamentous ''Bacillus subtilis'' cells]] and [[Team:Newcastle/glue|levansucrose]]. [[Team:Newcastle/Urease| CaCO<sub>3</sub>]] expands at the same rate as concrete, making it the ideal filler. A filamentous cell mesh provides reinforcement. Levansucrose glues CaCO<sub>3</sub> and filamentous cells in place. | BacillaFilla swims deep into the microcracks. Repair is effected by production of CaCO<sub>3</sub>, [[Team:Newcastle/Filamentous_Cells| filamentous ''Bacillus subtilis'' cells]] and [[Team:Newcastle/glue|levansucrose]]. [[Team:Newcastle/Urease| CaCO<sub>3</sub>]] expands at the same rate as concrete, making it the ideal filler. A filamentous cell mesh provides reinforcement. Levansucrose glues CaCO<sub>3</sub> and filamentous cells in place. | ||
''B. subtilis'' 168 sporulates, making it ideal for storage and transportation. The cells are naturally tolerant to concrete's high pH. We repaired ''B. subtilis'' 168's defective [[Team:Newcastle/Swarming|swrA and sfp ]], regaining motility. At the end of the crack the quorum sensing peptide [[Team:Newcastle/End_of_crack_%26_signalling_system|subtilin]] triggers a co-ordinated population response from a [[Team:Newcastle/End_of_crack_%26_signalling_system#2008Brick|subtilin-inducible promoter]]. Upregulating SR1 and rocF promotes arginine and urea production, increasing exogenous CaCO<sub>3</sub> deposition. Over-producing YneA induces the filamentous cell phenotype, while SacB converts extracellular sucrose to levansucrose glue. | ''B. subtilis'' 168 sporulates, making it ideal for storage and transportation. The cells are naturally tolerant to concrete's high pH. We repaired ''B. subtilis'' 168's defective [[Team:Newcastle/Swarming|swrA and sfp ]], regaining motility. At the end of the crack the quorum sensing peptide [[Team:Newcastle/End_of_crack_%26_signalling_system|subtilin]] triggers a co-ordinated population response from a [[Team:Newcastle/End_of_crack_%26_signalling_system#2008Brick|subtilin-inducible promoter]]. Upregulating SR1 and rocF promotes arginine and urea production, increasing exogenous CaCO<sub>3</sub> deposition. Over-producing YneA induces the filamentous cell phenotype, while SacB converts extracellular sucrose to levansucrose glue. |
Revision as of 16:31, 25 October 2010
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Contents |
iGEM Medal Requirements
Below are the requirements for each of the medals! As each requirement is completed by Team Newcastle 2010 they will be in bold and smiley faced :-)!!!
Bronze Award
The requirements to earn a Bronze Medal are:
-
BRONZE ONE:We have registered the team, and hope to continue having a great summer, and are looking forward to the Jamboree! :-) -
BRONZE TWO:Successfully complete and submit a Project Summary form.- Due September 20: see [1].
- Project Abstract:
BacillaFilla, an engineered Bacillus subtilis, aims to repair microcracks in concrete, which can cause catastrophic structural failure. BacillaFilla would be applied to structures by spraying onto their surface. BacillaFilla swims deep into the microcracks. Repair is effected by production of CaCO3, filamentous Bacillus subtilis cells and levansucrose. CaCO3 expands at the same rate as concrete, making it the ideal filler. A filamentous cell mesh provides reinforcement. Levansucrose glues CaCO3 and filamentous cells in place. B. subtilis 168 sporulates, making it ideal for storage and transportation. The cells are naturally tolerant to concrete's high pH. We repaired B. subtilis 168's defective swrA and sfp , regaining motility. At the end of the crack the quorum sensing peptide subtilin triggers a co-ordinated population response from a subtilin-inducible promoter. Upregulating SR1 and rocF promotes arginine and urea production, increasing exogenous CaCO3 deposition. Over-producing YneA induces the filamentous cell phenotype, while SacB converts extracellular sucrose to levansucrose glue.
-
BRONZE THREE:We have created and shared a description of our team's project on the iGEM wiki! :-) - BRONZE FOUR: Present a Poster and Talk at the iGEM Jamboree
- BRONZE FIVE: Enter information detailing at least one new standard BioBrick Part or Device in the Registry of Parts
- Entered information for each new part or device should at least include primary nucleic acid sequence, description of function, authorship, any relevant safety notes, and an acknowledgement of sources and references. E.g. [http://parts.mit.edu/registry/index.php/Part:BBa_J45004 BBa_J45004].
- Teams are currently expected to design and contribute standard biological parts that conform to the accepted BioBrick standards for physical assembly. Non-BioBrick parts will not be recognized by iGEM 2010 judges unless they have specific approval. The two specific BioBrick physical assembly schemes that the judges will recognize by default are (i) Tom Knight's [http://dspace.mit.edu/handle/1721.1/21168 original assembly standard] and (ii) Ira Phillips [http://dspace.mit.edu/handle/1721.1/32535 fusion assembly standard].
- [Special Note. A discussion has been initiated by the BioBricks Standards Working Group to consider updating the BioBrick assembly standard in time for June 1. Check back for any updates on acceptable BioBrick assembly standards.]
- Any new Devices that are based on gene expression are expected to conform to the PoPS device boundary standard. See chapter 3 of the book, [http://openwetware.org/wiki/Adventures Adventures in Synthetic Biology], for more information about common signal carriers and PoPS.
- What we have done so far: We have entered details (coding sequences) of most of the parts we are using into the registry. There are still some parts to enter. We are yet to include references, safety notes and further details.
- BRONZE SIX: Submit DNA for at least one new BioBrick Part or Device to the Registry of Parts.
- The submitted DNA must be associated with a Part or Device for which you have entered information describing the part or device, and must conform to the BioBrick standards for Parts or Devices (see above).
- IPTG-inducible filamentous cell formation part has had DNA sent to the registry
Silver Award
The requirements to earn a Silver Medal, in addition to the Bronze Medal requirements, are:
- SILVER ONE: Demonstrate that at least one new BioBrick Part or Device of your own design and construction works as Characterisation of the IPTG-inducible filamentous cell formation part
- SILVER TWO: Characterize the operation of at least one new BioBrick Part or Device and enter this information on the Parts or Device page via the Registry of Parts [http://partsregistry.org/Part:BBa_K302012:Experience| IPTG-inducible filamentous cell formation part: BBa_K302012]
Gold Award
The requirements to earn a Gold Medal, in addition to the Silver Medal requirements, are any one OR more of the following:
- GOLD ONE: Characterize or improve an existing BioBrick Part or Device and enter this information back on the Registry.
- We aim to improve the lacI repressible promoter pSpacoid, to create hyperspankoid and we aim to characterise this by the 27th of September.
- GOLD TWO: Help another iGEM team by, for example, characterizing a part, debugging a construct, or modeling or simulating their system.
- GOLD THREE: Develop and document a new technical standard that supports the (i) design of BioBrick Parts or Devices, or (ii) construction of BioBrick Parts or Devices, or (iii) characterization of BioBrick Parts or Devices, or (iv) analysis, modeling, and simulation of BioBrick Parts or Devices, or (v) sharing BioBrick Parts or Devices, either via physical DNA or as information via the internet.We investigate the benefits of an e-Science approach, with a focus on workflows, to synthetic biology.
- GOLD FOUR: Outline and detail a new approach to an issue of Human Practice in synthetic biology as it relates to your project, such as safety, security, ethics, or ownership, sharing, and innovation.
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