Team:Heidelberg/Modeling/miBSdesigner

From 2010.igem.org

(Difference between revisions)
Line 15: Line 15:
div.suboption {text-indent:80px;}
div.suboption {text-indent:80px;}
div.clear {clear: both;}
div.clear {clear: both;}
-
div.reswrap2 {margin:auto; text-align:left; display:none; font-size:12pt;}
+
div.reswrap2 {width: 270px; margin:auto; text-align:left; display:none; font-size:12pt;}
.variaresult {font-size: 12pt; color: #11526f; font-weight: bold;}
.variaresult {font-size: 12pt; color: #11526f; font-weight: bold;}
</style>
</style>
Line 124: Line 124:
<img style="border-width: 0px;" src="https://static.igem.org/mediawiki/2010/c/c0/MiBSdesigner.png" width="260" height="147" />
<img style="border-width: 0px;" src="https://static.igem.org/mediawiki/2010/c/c0/MiBSdesigner.png" width="260" height="147" />
<br><br>
<br><br>
-
</div>
+
</div><br><br>
<div class="reswrap2" id="resultdiv">
<div class="reswrap2" id="resultdiv">
<div class="variaresult">Name of primer 1</div>
<div class="variaresult">Name of primer 1</div>

Revision as of 15:18, 25 October 2010

BS designer for miRNA
Sequence name

microRNA Sequence ( 5' -> 3')

Spacer (inert sequence)


Perfect Binding Site
Imperfect Binding Site with 4 nt bulge (9-12)
Personalized Binding Site

Seed sequence (1-8)
Customized MISMATCH position

Supplementary region
Customized MATCH positions (input numbers from 9 to 22)


Modify AU content
A in position -1
A in position 0
A in position 1
A in position 8
A in position 9
A in position 10


Sticky ends for integration in plasmid:
Customized: 5' 3'



Construction of microRNA binding sites





Name of primer 1


Sequence of primer 1


Name of primer 2


Sequence of primer 2