Team:Heidelberg/Modeling/miBSdesigner

From 2010.igem.org

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Construction of microRNA binding sites </span>
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Revision as of 14:45, 25 October 2010

BS designer for miRNA
Sequence name

microRNA Sequence ( 5' -> 3')

Spacer (inert sequence)


Perfect Binding Site
Imperfect Binding Site with 4 nt bulge (9-12)
Personalized Binding Site

Seed sequence (1-8)
Customized MISMATCH position

Supplementary region
Customized MATCH positions (input numbers from 9 to 22)


Modify AU content
A in position -1
A in position 0
A in position 1
A in position 8
A in position 9
A in position 10


Sticky ends for integration in plasmid:
Customized: 5' 3'




Construction of microRNA binding sites
Name of primer 1


Sequence of primer 1


Name of primer 2


Sequence of primer 2

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