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Team:Newcastle/9 July 2010
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=LacI BioBrick Construction= | =LacI BioBrick Construction= | ||
Revision as of 14:39, 25 October 2010
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Contents |
LacI BioBrick Construction
Aims
- To use PCR to extract lacI (promoter, ribosome-binding site (RBS) & coding sequence (CDS)) from plasmid pMutin4 and ligate into vector pSB1AT3 in front of red fluorescent protein (RFP).
Materials
- Competent E. coli (DH5alpha strain)
- Ligation of lacI into pSB1AT3
Protocol
- Transformation of E. coli DH5α with pSB1AT3 containing lacI insert.
Inference
- Ecoli DH5alpha is used as a bioreactor to replicate our plasmids to create a large stock ready for extraction from cells.
   
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