Team:Heidelberg/Modeling/miBSdesigner

From 2010.igem.org

(Difference between revisions)
Line 131: Line 131:
<div class="reswrap2" id="resultdiv">
<div class="reswrap2" id="resultdiv">
<html>
<html>
-
                 <br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br>
+
                 <br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br>
<div class="variaresult">Name of primer 1</div>
<div class="variaresult">Name of primer 1</div>
<textarea name="NamA" cols=30 rows=1 id="NamA" wrap=SOFT></textarea><br><br>
<textarea name="NamA" cols=30 rows=1 id="NamA" wrap=SOFT></textarea><br><br>

Revision as of 13:16, 25 October 2010

BS designer for miRNA


Construction of microRNA binding sites
Sequence name

microRNA Sequence ( 5' -> 3')

Spacer (inert sequence)

Perfect Binding Site
Imperfect Binding Site with 4 nt bulge (9-12)
Personalized Binding Site

Seed sequence (1-8)
Customized MISMATCH position

Supplementary region
Customized MATCH positions (input numbers from 9 to 22)


Modify AU content
A in position -1
A in position 0
A in position 1
A in position 8
A in position 9
A in position 10


Sticky ends for integration in plasmid:
Customized: 5' 3'
























Name of primer 1


Sequence of primer 1


Name of primer 2


Sequence of primer 2

Retrieved from "http://2010.igem.org/Team:Heidelberg/Modeling/miBSdesigner"